{"_buckets": {"deposit": "575b1403-46f5-46b4-a743-5d758093c070"}, "_deposit": {"id": "2525", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "2525"}, "status": "published"}, "_oai": {"id": "oai:niigata-u.repo.nii.ac.jp:00002525"}, "item_5_biblio_info_6": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2003-06", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "3", "bibliographicPageEnd": "194", "bibliographicPageStart": "187", "bibliographicVolumeNumber": "20", "bibliographic_titles": [{"bibliographic_title": "Plant biotechnology"}, {"bibliographic_title": "Plant biotechnology", "bibliographic_titleLang": "en"}]}]}, "item_5_description_4": {"attribute_name": "\u6284\u9332", "attribute_value_mlt": [{"subitem_description": "Effects of phosphate on the Ca2+ uptake and the sucrose-controlled secretion of \u03b1-amylase molecules in cultured rice cells were investigated. Phosphate markedly stimulated Ca2+ uptake into rice cells, particularly at the outer cell layer of the cell cluster. Phosphate also increased the synthesis and extracellular liberation of \u03b1-amylase II-4 in sucrose-supplemented cells. The distribution pattern of enzyme in rice cell clusters induced by phosphate was similar to that of Ca2+ uptake. Phosphate did not increase the level of mRNA of \u03b1-amylase II-4, indicating that phosphate stimulates the translation and posttranslational secretory processes of \u03b1-amylase II-4 in the presence of sucrose. Furthermore, phosphate enhanced both the Ca2+ uptake and \u03b1-amylase II-4 synthesis in the microsomes. These results strongly suggested that the ratio of phosphate to sugar is important for regulating the Ca2+ uptake, and that phosphate and sugar precisely coordinate the Ca2+-mediated synthesis and extracellular liberation of \u03b1-amylase II-4.", "subitem_description_type": "Abstract"}]}, "item_5_publisher_7": {"attribute_name": "\u51fa\u7248\u8005", "attribute_value_mlt": [{"subitem_publisher": "\u65e5\u672c\u690d\u7269\u7d30\u80de\u5206\u5b50\u751f\u7269\u5b66\u4f1a"}]}, "item_5_select_19": {"attribute_name": "\u8457\u8005\u7248\u30d5\u30e9\u30b0", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_5_source_id_11": {"attribute_name": "\u66f8\u8a8c\u30ec\u30b3\u30fc\u30c9ID", "attribute_value_mlt": [{"subitem_source_identifier": "AA11250821", "subitem_source_identifier_type": "NCID"}]}, "item_5_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "13424580", "subitem_source_identifier_type": "ISSN"}]}, "item_creator": {"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Asatsuma, Satoru"}], "nameIdentifiers": [{"nameIdentifier": "37614", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Nanjo, Yohei"}], "nameIdentifiers": [{"nameIdentifier": "37615", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kashem, Mohammad A."}], "nameIdentifiers": [{"nameIdentifier": "37616", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Itoh, Kimiko"}], "nameIdentifiers": [{"nameIdentifier": "37617", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Hori, Hidetaka"}], "nameIdentifiers": [{"nameIdentifier": "37618", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Ohshima, Masahiro"}], "nameIdentifiers": [{"nameIdentifier": "37619", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Mitsui, Toshiaki"}], "nameIdentifiers": [{"nameIdentifier": "37620", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2019-07-29"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "ZL_20(3)_187-194.pdf", "filesize": [{"value": "1.8 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 1800000.0, "url": {"label": "ZL_20(3)_187-194.pdf", "url": "https://niigata-u.repo.nii.ac.jp/record/2525/files/ZL_20(3)_187-194.pdf"}, "version_id": "a7ba5314-56be-4c62-af4f-6daab658892c"}]}, "item_keyword": {"attribute_name": "\u30ad\u30fc\u30ef\u30fc\u30c9", "attribute_value_mlt": [{"subitem_subject": "\u03b1-amylase", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Ca2+", "subitem_subject_scheme": "Other"}, {"subitem_subject": "cell culture", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Oryza sativa L.", "subitem_subject_scheme": "Other"}, {"subitem_subject": "phosphates", "subitem_subject_scheme": "Other"}, {"subitem_subject": "sucrose", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Phosphate modulates Ca2+ uptake and \u03b1-amylase secretion in suspension-cultured cells of rice", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "Phosphate modulates Ca2+ uptake and \u03b1-amylase secretion in suspension-cultured cells of rice"}, {"subitem_title": "Phosphate modulates Ca2+ uptake and \u03b1-amylase secretion in suspension-cultured cells of rice", "subitem_title_language": "en"}]}, "item_type_id": "5", "owner": "1", "path": ["453/454", "485/486/487"], "permalink_uri": "http://hdl.handle.net/10191/17593", "pubdate": {"attribute_name": "\u516c\u958b\u65e5", "attribute_value": "2012-04-04"}, "publish_date": "2012-04-04", "publish_status": "0", "recid": "2525", "relation": {}, "relation_version_is_last": true, "title": ["Phosphate modulates Ca2+ uptake and \u03b1-amylase secretion in suspension-cultured cells of rice"], "weko_shared_id": null}