WEKO3
アイテム
雑種肝細胞株におけるA型肝炎ウイルスの増殖
http://hdl.handle.net/10191/36262
http://hdl.handle.net/10191/36262822620b6-ba89-4d15-9c3c-db28f37257e3
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
101(1)_46-57.pdf (3.0 MB)
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| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2016-01-05 | |||||
| タイトル | ||||||
| タイトル | 雑種肝細胞株におけるA型肝炎ウイルスの増殖 | |||||
| タイトル | ||||||
| タイトル | 雑種肝細胞株におけるA型肝炎ウイルスの増殖 | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | jpn | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | hepatitis A virus | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | hybrid liver cell lines | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | marmoset | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Chinese hamster ovary cells | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Vero cells | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | A型肝炎ウイルス | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | 雑種肝細胞株 | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | マーモセット | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | チャイニーズハムスターオバリー細胞 | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Vero細胞 | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | departmental bulletin paper | |||||
| その他のタイトル | ||||||
| その他のタイトル | Propagation of Hepatitis A Virus in Hybrid Liver Cell Lines | |||||
| 著者 |
芦田, 雅彦
× 芦田, 雅彦 |
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| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 145515 | |||||
| 姓名 | Ashida, Masahiko | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Preferential growth of HAV （hepatitis A vivus ）in the primate liver tissue is already known. To obtain established cell lines with a high susceptibility to HAV, marmoset liver cells were fused with CHO （Chinese hamster ovary） or Vero （a derivative of African green monkey kidney cells）cells by a cell fusion technique with polyethylene glycol. The experiment yielded 6 hybrid cell lines（C.ja/CHO－1 to －6）between the liver and CHO cells, and 4 cell lines （S.la/Ve－1 to －4）between the liver and Vero cells. Hybrid cell lines obtained were assessed for their susceptibility by inoculating two sources of HAV, one from an infected marmoset liver tissue（liver tissue virus）and another from a fecal specimen of patient, where the virus growth was monitored by RIA （radioimmunoassy）and IFA （immunofluorescent antibody technique）, giving the following results. 1）Of 6 hybrid cell lines comprising the liver and CHO cells, three （C.ja/CHO－1, －3, and －4）were subjected to the HAV infection with the fecal virus, whereby two （C.ja/CHO－3, －4） of them were found to develop significant activities of the viral antigen transiently at 10 to 12th passages of cells. 2）Of 4 hybrid cell lines comprising the liver and Vero cells, three（S.la/Ve－1, －3, and －4）were equally susceptible te HAV, giving maximal activities of the viral antigen at 6 to 8 weeks post infection （pi） with the liver tissue virus and at 10 to 12 weeks pi with the fecal virus. 3）In S.la/Ve－1 cells, a representative of the susceptible hybrid cell lines, passage of HAV was commenced with a ten fold concentrate of the liver tissue virus, resulting in the development of plateau level activities of the viral antigen at 4 weeks pi at the initial passage and at 1 week pi after the 6th passage consistently. 4）HAV－infected S.la/Ve－1 cells did not develop CPE （cytopathic effect） and could be subcultured in an equal efficiency to that of the uninfected counterparts, retaining the maximal growth of HAV. 5）In S.la/Ve－1 cells, the cell culture-adapted HAV grew to the plateau level at 7 days pi giving an infectivity of 10^<8.5>TCID_<50>/ml, where the virus particles were confirmed in the cellular extracts in abundance by the immune electron microscopy. Taken these findings together, S.la/Ve－1, －3, and －4 cells were considered to be well susceptible to HAV and quite useful in the basic and clinical studies of HAV. | |||||
| 書誌情報 |
新潟医学会雑誌 en : 新潟医学会雑誌 巻 101, 号 1, p. 46-57, 発行日 1987-01 |
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| 出版者 | ||||||
| 出版者 | 新潟医学会 | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 00290440 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AN00182415 | |||||
| 著者版フラグ | ||||||
| 値 | publisher | |||||
