{"created":"2021-03-01T06:29:26.958841+00:00","id":24122,"links":{},"metadata":{"_buckets":{"deposit":"9c29c622-537b-4f7a-8d09-17b2411d3289"},"_deposit":{"id":"24122","owners":[],"pid":{"revision_id":0,"type":"depid","value":"24122"},"status":"published"},"_oai":{"id":"oai:niigata-u.repo.nii.ac.jp:00024122"},"item_7_alternative_title_1":{"attribute_name":"\u305d\u306e\u4ed6\u306e\u30bf\u30a4\u30c8\u30eb","attribute_value_mlt":[{"subitem_alternative_title":"Characterization of the surface antigens on human mononuclear cells to which anti-myelin associated glycoprotein (MAG) monoclonal antibodies bind"}]},"item_7_biblio_info_6":{"attribute_name":"\u66f8\u8a8c\u60c5\u5831","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1987-06","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"6","bibliographicPageEnd":"389","bibliographicPageStart":"382","bibliographicVolumeNumber":"101","bibliographic_titles":[{"bibliographic_title":"\u65b0\u6f5f\u533b\u5b66\u4f1a\u96d1\u8a8c"},{"bibliographic_title":"\u65b0\u6f5f\u533b\u5b66\u4f1a\u96d1\u8a8c","bibliographic_titleLang":"en"}]}]},"item_7_description_4":{"attribute_name":"\u6284\u9332","attribute_value_mlt":[{"subitem_description":"Three (GC-J4, MC-P2 and MC-P4) out of five murine monoclonal antibodies (mAbs) to myelin-associated glycoprotein (MAG) bound to human mononuclear cells (MNC). The results of the competitive inhibition assay suggested that MC-P2 and MC-P4 bind directly to or close to the Leu 7 epitope, and that GC-J4 binds to the epitope which is distinct from the Leu 7 epitope. The electrophoretic patterns of immunoprecipitates with GC-J4, MC-P2 and anti-Leu 7 from detergent lysates of surface labeled human MNC were very similar. The target molecules of anti-Leu 7, MC-P2 and GC-J4 have apparent molecular weights of 205, 170, 150, 135, 110, 85, 65 and 55 kDa, and they are monomeric or noncovalently associated proteins. The patterns of immunoprecipitates with MC-P4 were slightly different from those with anti-Leu 7 and MC-P2. The implications of these results are as follows: 1) The mAb with specificity to the carbohydrate part of MAG reacts with the Leu 7-reactive molecule on human MNC. 2) At least two epitopes detected by anti-MAG mAbs coexist on the surface molecules with various apparent molecular weights. 3) The fine antigenic specificity of MC-P4 is different from those of anti-Leu 7 or MC-P2.","subitem_description_type":"Abstract"}]},"item_7_full_name_3":{"attribute_name":"\u8457\u8005\u5225\u540d","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"144533","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Arai, Motomi"}]}]},"item_7_publisher_7":{"attribute_name":"\u51fa\u7248\u8005","attribute_value_mlt":[{"subitem_publisher":"\u65b0\u6f5f\u533b\u5b66\u4f1a"}]},"item_7_select_19":{"attribute_name":"\u8457\u8005\u7248\u30d5\u30e9\u30b0","attribute_value_mlt":[{"subitem_select_item":"publisher"}]},"item_7_source_id_11":{"attribute_name":"\u66f8\u8a8c\u30ec\u30b3\u30fc\u30c9ID","attribute_value_mlt":[{"subitem_source_identifier":"AN00182415","subitem_source_identifier_type":"NCID"}]},"item_7_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"00290440","subitem_source_identifier_type":"ISSN"}]},"item_creator":{"attribute_name":"\u8457\u8005","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"\u8352\u4e95, \u5143\u7f8e"}],"nameIdentifiers":[{"nameIdentifier":"144532","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"\u30d5\u30a1\u30a4\u30eb\u60c5\u5831","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-08-19"}],"displaytype":"detail","filename":"101(6)_382-389.pdf","filesize":[{"value":"1.0 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"101(6)_382-389.pdf","url":"https://niigata-u.repo.nii.ac.jp/record/24122/files/101(6)_382-389.pdf"},"version_id":"96f12fb7-bcf7-433c-bb63-c3775810d18e"}]},"item_keyword":{"attribute_name":"\u30ad\u30fc\u30ef\u30fc\u30c9","attribute_value_mlt":[{"subitem_subject":"monoclonal antibody","subitem_subject_scheme":"Other"},{"subitem_subject":"myelin-associated glycoprotein","subitem_subject_scheme":"Other"},{"subitem_subject":"mononuclear cells","subitem_subject_scheme":"Other"},{"subitem_subject":"surface marker","subitem_subject_scheme":"Other"},{"subitem_subject":"\u30e2\u30ce\u30af\u30ed\u30fc\u30ca\u30eb\u6297\u4f53","subitem_subject_scheme":"Other"},{"subitem_subject":"\u30df\u30a8\u30ea\u30f3\u7cd6\u86cb\u767d","subitem_subject_scheme":"Other"},{"subitem_subject":"\u30ea\u30f3\u30d1\u7403","subitem_subject_scheme":"Other"},{"subitem_subject":"\u8868\u9762\u6297\u539f","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"\u8a00\u8a9e","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"\u8cc7\u6e90\u30bf\u30a4\u30d7","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"\u6297Myelin-associated glycoprotein(MAG)\u30e2\u30ce\u30af\u30ed\u30fc\u30ca\u30eb\u6297\u4f53\u3068\u7d50\u5408\u3059\u308b\u30d2\u30c8\u306e\u30ea\u30f3\u30d1\u7403\u8868\u9762\u6297\u539f\u306e\u89e3\u6790","item_titles":{"attribute_name":"\u30bf\u30a4\u30c8\u30eb","attribute_value_mlt":[{"subitem_title":"\u6297Myelin-associated glycoprotein(MAG)\u30e2\u30ce\u30af\u30ed\u30fc\u30ca\u30eb\u6297\u4f53\u3068\u7d50\u5408\u3059\u308b\u30d2\u30c8\u306e\u30ea\u30f3\u30d1\u7403\u8868\u9762\u6297\u539f\u306e\u89e3\u6790"},{"subitem_title":"\u6297Myelin-associated glycoprotein(MAG)\u30e2\u30ce\u30af\u30ed\u30fc\u30ca\u30eb\u6297\u4f53\u3068\u7d50\u5408\u3059\u308b\u30d2\u30c8\u306e\u30ea\u30f3\u30d1\u7403\u8868\u9762\u6297\u539f\u306e\u89e3\u6790","subitem_title_language":"en"}]},"item_type_id":"7","owner":"1","path":["453/456","471/537/538/1233"],"pubdate":{"attribute_name":"\u516c\u958b\u65e5","attribute_value":"2016-01-07"},"publish_date":"2016-01-07","publish_status":"0","recid":"24122","relation_version_is_last":true,"title":["\u6297Myelin-associated glycoprotein(MAG)\u30e2\u30ce\u30af\u30ed\u30fc\u30ca\u30eb\u6297\u4f53\u3068\u7d50\u5408\u3059\u308b\u30d2\u30c8\u306e\u30ea\u30f3\u30d1\u7403\u8868\u9762\u6297\u539f\u306e\u89e3\u6790"],"weko_creator_id":"1","weko_shared_id":null},"updated":"2021-03-01T12:39:33.086157+00:00"}