@article{oai:niigata-u.repo.nii.ac.jp:00023579,
 author = {原, 秀範},
 issue = {3},
 journal = {新潟医学会雑誌, 新潟医学会雑誌},
 month = {Mar},
 note = {S. la/Ve-1 cells, a hybrid cell line between marmoset liver and Vero cells, have been reported to be highly susceptible to HAV (hepatitis A virus). To examine the susceptibility of the cell line to field HAVs and compare the susceptibility with that of other monkey kidney derived cell lines, S.la/Ve-1 cells, together with BSC-1, LLC-MK_2, BGM and Vero cells, were inoculated with 11 human fecal HAV specimens (5 positive and 6 negative for HAV Ag) and development of the viral antigen in cells was monitored by radioimmunoassay (RIA) and immune fluorescent antibody technique (IFA). In addition, virus growth experiments were conducted with a tissue culture-adapted HAV to assess the kinetics of development of virus infectivity in these various cell lines. Results obtained are summarized below. 1) Of the 11 fecal specimens, 7(5 positive and 2 negative for HAV Ag) induced significant elevation of the virus Ag activity in S. la/Ve-1 cells. 2) Of the 7 HAV isolates 6 were confirmed to be transmissible to the fresh S.la/Ve-1 cells by blind passage. 3) Of the 4 monkey kidney-derived cell lines, BSC-1 and LLC-MK_2 cells were inoculated with 6 fecal specimens (3 positive and 3 negative for HAV Ag), and BCM and Vero cells were inoculated with 4 fecal specimens (2 positive and 2 negative for HAV Ag), resulting in a single isolation of HAV by BGM cells from a HAV Ag-positive specimens. 4) In the subcultures of persistently HAV-infected S. la/V-1 cells, a plateau level of HAV Ag activities was reached by T. T. isolate on the 1st passage and by Ku-1～-4 isolates on the 10th passage, indicating that the kinetics of cell culture adaptation of HAV was distinctive by isolates. 5) In the growth experiment of T. T. isolate in various cell lines, plateau level titers of virus infectivity were attained at 8 days post infection, when the virus infectivity titers were 10^<7.5-8.25> TCID_<50>/ml in S.la/Ve-1, BGM and BSC-1 cells and 10^<5.5>  TCID_<50>/ml in LLC-MK_2 and Vero cells. 6) In S.la/Ve-1 cells, the growth kinetics of cell culture-adapted T. T. isolate were accelerated in the subculture passage of persistently infected cells giving a plateau level infectivity (10^<7.5> TCID_<50>/ml) at 6 days post infection, as compared with that in the inoculation passage of the virus, where the plateau level infectivity resulted at 8 days post infection. Taken these findings together, S.la/Ve-1 cell line, in comparison with other conventional cell lines established from monkey kidney cells, was considered to be highly susceptible to the human fecal HAVs and to be quite useful for the virus isolation from clinical specimens. In addition, the cell line was revealed to be advantageous for obtaining lots of virus and viral antigens in persistent infection.},
 pages = {173--184},
 title = {雑種サル肝細胞株によるヒト糞便A型肝炎ウイルスの分離と増殖},
 volume = {102},
 year = {1988}
}