WEKO3
アイテム
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To examine the susceptibility of the cell line to field HAVs and compare the susceptibility with that of other monkey kidney derived cell lines, S.la/Ve-1 cells, together with BSC-1, LLC-MK_2, BGM and Vero cells, were inoculated with 11 human fecal HAV specimens (5 positive and 6 negative for HAV Ag) and development of the viral antigen in cells was monitored by radioimmunoassay (RIA) and immune fluorescent antibody technique (IFA). In addition, virus growth experiments were conducted with a tissue culture-adapted HAV to assess the kinetics of development of virus infectivity in these various cell lines. Results obtained are summarized below. 1) Of the 11 fecal specimens, 7(5 positive and 2 negative for HAV Ag) induced significant elevation of the virus Ag activity in S. la/Ve-1 cells. 2) Of the 7 HAV isolates 6 were confirmed to be transmissible to the fresh S.la/Ve-1 cells by blind passage. 3) Of the 4 monkey kidney-derived cell lines, BSC-1 and LLC-MK_2 cells were inoculated with 6 fecal specimens (3 positive and 3 negative for HAV Ag), and BCM and Vero cells were inoculated with 4 fecal specimens (2 positive and 2 negative for HAV Ag), resulting in a single isolation of HAV by BGM cells from a HAV Ag-positive specimens. 4) In the subcultures of persistently HAV-infected S. la/V-1 cells, a plateau level of HAV Ag activities was reached by T. T. isolate on the 1st passage and by Ku-1~-4 isolates on the 10th passage, indicating that the kinetics of cell culture adaptation of HAV was distinctive by isolates. 5) In the growth experiment of T. T. isolate in various cell lines, plateau level titers of virus infectivity were attained at 8 days post infection, when the virus infectivity titers were 10^\u003c7.5-8.25\u003e TCID_\u003c50\u003e/ml in S.la/Ve-1, BGM and BSC-1 cells and 10^\u003c5.5\u003e TCID_\u003c50\u003e/ml in LLC-MK_2 and Vero cells. 6) In S.la/Ve-1 cells, the growth kinetics of cell culture-adapted T. T. isolate were accelerated in the subculture passage of persistently infected cells giving a plateau level infectivity (10^\u003c7.5\u003e TCID_\u003c50\u003e/ml) at 6 days post infection, as compared with that in the inoculation passage of the virus, where the plateau level infectivity resulted at 8 days post infection. Taken these findings together, S.la/Ve-1 cell line, in comparison with other conventional cell lines established from monkey kidney cells, was considered to be highly susceptible to the human fecal HAVs and to be quite useful for the virus isolation from clinical specimens. 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雑種サル肝細胞株によるヒト糞便A型肝炎ウイルスの分離と増殖
http://hdl.handle.net/10191/40644
http://hdl.handle.net/10191/40644c0dc7f87-32fa-4d90-b08f-fd6b0ae5d35c
名前 / ファイル | ライセンス | アクション |
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2016-04-04 | |||||
タイトル | ||||||
タイトル | 雑種サル肝細胞株によるヒト糞便A型肝炎ウイルスの分離と増殖 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | 雑種サル肝細胞株によるヒト糞便A型肝炎ウイルスの分離と増殖 | |||||
言語 | ||||||
言語 | jpn | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | hepatitis A virus | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | hybrid liver cell line | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | primary isolation of virus | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | virus growth | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | monkey kidney-derived cell lines | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | A型肝炎ウイルス | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 雑種サル肝細胞株 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | ウイルス直接分離 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | ウイルス増殖 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | サル腎由来細胞株 | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | departmental bulletin paper | |||||
その他のタイトル | ||||||
その他のタイトル | Primary Isolation and Propagation of Hepatitis A Virus in Hybrid Liver Cell Line | |||||
著者 |
原, 秀範
× 原, 秀範 |
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著者別名 | ||||||
識別子 | 141828 | |||||
識別子Scheme | WEKO | |||||
姓名 | Hara, Hidenori | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | S. la/Ve-1 cells, a hybrid cell line between marmoset liver and Vero cells, have been reported to be highly susceptible to HAV (hepatitis A virus). To examine the susceptibility of the cell line to field HAVs and compare the susceptibility with that of other monkey kidney derived cell lines, S.la/Ve-1 cells, together with BSC-1, LLC-MK_2, BGM and Vero cells, were inoculated with 11 human fecal HAV specimens (5 positive and 6 negative for HAV Ag) and development of the viral antigen in cells was monitored by radioimmunoassay (RIA) and immune fluorescent antibody technique (IFA). In addition, virus growth experiments were conducted with a tissue culture-adapted HAV to assess the kinetics of development of virus infectivity in these various cell lines. Results obtained are summarized below. 1) Of the 11 fecal specimens, 7(5 positive and 2 negative for HAV Ag) induced significant elevation of the virus Ag activity in S. la/Ve-1 cells. 2) Of the 7 HAV isolates 6 were confirmed to be transmissible to the fresh S.la/Ve-1 cells by blind passage. 3) Of the 4 monkey kidney-derived cell lines, BSC-1 and LLC-MK_2 cells were inoculated with 6 fecal specimens (3 positive and 3 negative for HAV Ag), and BCM and Vero cells were inoculated with 4 fecal specimens (2 positive and 2 negative for HAV Ag), resulting in a single isolation of HAV by BGM cells from a HAV Ag-positive specimens. 4) In the subcultures of persistently HAV-infected S. la/V-1 cells, a plateau level of HAV Ag activities was reached by T. T. isolate on the 1st passage and by Ku-1~-4 isolates on the 10th passage, indicating that the kinetics of cell culture adaptation of HAV was distinctive by isolates. 5) In the growth experiment of T. T. isolate in various cell lines, plateau level titers of virus infectivity were attained at 8 days post infection, when the virus infectivity titers were 10^<7.5-8.25> TCID_<50>/ml in S.la/Ve-1, BGM and BSC-1 cells and 10^<5.5> TCID_<50>/ml in LLC-MK_2 and Vero cells. 6) In S.la/Ve-1 cells, the growth kinetics of cell culture-adapted T. T. isolate were accelerated in the subculture passage of persistently infected cells giving a plateau level infectivity (10^<7.5> TCID_<50>/ml) at 6 days post infection, as compared with that in the inoculation passage of the virus, where the plateau level infectivity resulted at 8 days post infection. Taken these findings together, S.la/Ve-1 cell line, in comparison with other conventional cell lines established from monkey kidney cells, was considered to be highly susceptible to the human fecal HAVs and to be quite useful for the virus isolation from clinical specimens. In addition, the cell line was revealed to be advantageous for obtaining lots of virus and viral antigens in persistent infection. | |||||
書誌情報 |
新潟医学会雑誌 en : 新潟医学会雑誌 巻 102, 号 3, p. 173-184, 発行日 1988-03 |
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出版者 | ||||||
出版者 | 新潟医学会 | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 00290440 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AN00182415 | |||||
著者版フラグ | ||||||
値 | publisher |