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To examine the susceptibility of the cell line to field HAVs and compare the susceptibility with that of other monkey kidney derived cell lines, S.la/Ve-1 cells, together with BSC-1, LLC-MK_2, BGM and Vero cells, were inoculated with 11 human fecal HAV specimens (5 positive and 6 negative for HAV Ag) and development of the viral antigen in cells was monitored by radioimmunoassay (RIA) and immune fluorescent antibody technique (IFA). In addition, virus growth experiments were conducted with a tissue culture-adapted HAV to assess the kinetics of development of virus infectivity in these various cell lines. 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  1. 0 資料タイプ別
  2. 03 紀要論文
  1. 250 大学院医歯学総合研究科(医)
  2. 20 紀要
  3. 02 新潟医学会雑誌
  4. 第102巻第3号

雑種サル肝細胞株によるヒト糞便A型肝炎ウイルスの分離と増殖

http://hdl.handle.net/10191/40644
http://hdl.handle.net/10191/40644
c0dc7f87-32fa-4d90-b08f-fd6b0ae5d35c
名前 / ファイル ライセンス アクション
102(3)_173-184.pdf 102(3)_173-184.pdf (2.9 MB)
Item type 紀要論文 / Departmental Bulletin Paper(1)
公開日 2016-04-04
タイトル
タイトル 雑種サル肝細胞株によるヒト糞便A型肝炎ウイルスの分離と増殖
タイトル
言語 en
タイトル 雑種サル肝細胞株によるヒト糞便A型肝炎ウイルスの分離と増殖
言語
言語 jpn
キーワード
主題Scheme Other
主題 hepatitis A virus
キーワード
主題Scheme Other
主題 hybrid liver cell line
キーワード
主題Scheme Other
主題 primary isolation of virus
キーワード
主題Scheme Other
主題 virus growth
キーワード
主題Scheme Other
主題 monkey kidney-derived cell lines
キーワード
主題Scheme Other
主題 A型肝炎ウイルス
キーワード
主題Scheme Other
主題 雑種サル肝細胞株
キーワード
主題Scheme Other
主題 ウイルス直接分離
キーワード
主題Scheme Other
主題 ウイルス増殖
キーワード
主題Scheme Other
主題 サル腎由来細胞株
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ departmental bulletin paper
その他のタイトル
その他のタイトル Primary Isolation and Propagation of Hepatitis A Virus in Hybrid Liver Cell Line
著者 原, 秀範

× 原, 秀範

WEKO 141827

原, 秀範

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著者別名
識別子 141828
識別子Scheme WEKO
姓名 Hara, Hidenori
抄録
内容記述タイプ Abstract
内容記述 S. la/Ve-1 cells, a hybrid cell line between marmoset liver and Vero cells, have been reported to be highly susceptible to HAV (hepatitis A virus). To examine the susceptibility of the cell line to field HAVs and compare the susceptibility with that of other monkey kidney derived cell lines, S.la/Ve-1 cells, together with BSC-1, LLC-MK_2, BGM and Vero cells, were inoculated with 11 human fecal HAV specimens (5 positive and 6 negative for HAV Ag) and development of the viral antigen in cells was monitored by radioimmunoassay (RIA) and immune fluorescent antibody technique (IFA). In addition, virus growth experiments were conducted with a tissue culture-adapted HAV to assess the kinetics of development of virus infectivity in these various cell lines. Results obtained are summarized below. 1) Of the 11 fecal specimens, 7(5 positive and 2 negative for HAV Ag) induced significant elevation of the virus Ag activity in S. la/Ve-1 cells. 2) Of the 7 HAV isolates 6 were confirmed to be transmissible to the fresh S.la/Ve-1 cells by blind passage. 3) Of the 4 monkey kidney-derived cell lines, BSC-1 and LLC-MK_2 cells were inoculated with 6 fecal specimens (3 positive and 3 negative for HAV Ag), and BCM and Vero cells were inoculated with 4 fecal specimens (2 positive and 2 negative for HAV Ag), resulting in a single isolation of HAV by BGM cells from a HAV Ag-positive specimens. 4) In the subcultures of persistently HAV-infected S. la/V-1 cells, a plateau level of HAV Ag activities was reached by T. T. isolate on the 1st passage and by Ku-1~-4 isolates on the 10th passage, indicating that the kinetics of cell culture adaptation of HAV was distinctive by isolates. 5) In the growth experiment of T. T. isolate in various cell lines, plateau level titers of virus infectivity were attained at 8 days post infection, when the virus infectivity titers were 10^<7.5-8.25> TCID_<50>/ml in S.la/Ve-1, BGM and BSC-1 cells and 10^<5.5> TCID_<50>/ml in LLC-MK_2 and Vero cells. 6) In S.la/Ve-1 cells, the growth kinetics of cell culture-adapted T. T. isolate were accelerated in the subculture passage of persistently infected cells giving a plateau level infectivity (10^<7.5> TCID_<50>/ml) at 6 days post infection, as compared with that in the inoculation passage of the virus, where the plateau level infectivity resulted at 8 days post infection. Taken these findings together, S.la/Ve-1 cell line, in comparison with other conventional cell lines established from monkey kidney cells, was considered to be highly susceptible to the human fecal HAVs and to be quite useful for the virus isolation from clinical specimens. In addition, the cell line was revealed to be advantageous for obtaining lots of virus and viral antigens in persistent infection.
書誌情報 新潟医学会雑誌
en : 新潟医学会雑誌

巻 102, 号 3, p. 173-184, 発行日 1988-03
出版者
出版者 新潟医学会
ISSN
収録物識別子タイプ ISSN
収録物識別子 00290440
書誌レコードID
収録物識別子タイプ NCID
収録物識別子 AN00182415
著者版フラグ
値 publisher
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