@article{oai:niigata-u.repo.nii.ac.jp:00022813,
 author = {長谷川, まこと},
 issue = {8},
 journal = {新潟医学会雑誌, 新潟医学会雑誌},
 month = {Aug},
 note = {Cholecystokinin (CCK), a brain gut peptide, which is assumed to be a neurotransmitter or a neuromodulater, may play an important role in mental and neurological function. The concentration of CCK in various regions of rat brains was determined by radioimmunoassay, and the receptor activity for ^3H-CCK in various areas of guinea pig brains was estimated. The effect of repeated administration of methamphetamine and haloperidol on the CCK concentration and CCK receptor were also studied. Administration of methamphetamine and haloperidol decreased CCK concentration in some areas of the brains, but the difference was not significant statistically. Administration of methamphetamine decreased CCK receptor binding (decreased receptor number) in the frontal cortex of guinea pig brains, which suggests that CCK is involved in some psychotic conditions caused by methamphetamine. Furthermore, using previously cloned CCK cDNA as a probe, the presence of the CCK gene in rat genomic DNA was demonstrated. Then, the CCK cDNA was applied to quantitate the CCKmRNA. Northern blot analysis showed that the size of CCKmRNA is about 850 nucleotides in length. Developmental changes and regional distribution of CCKmRNA in rat brain were also examined by dot blot hybridization. CCKmRNA was barely detectable in the fetal brain, but started to increase postnatally and attained a plateau level after 20-30 days. Further, the level of CCKmRNA was the highest in the frontal cortex and the lowest in the cerebellum, which correlated with the concentration of CCK.},
 pages = {659--666},
 title = {コレシストキニンの脳内動態 : 定量, 受容体解析, mRNAの測定},
 volume = {103},
 year = {1989}
}