@article{oai:niigata-u.repo.nii.ac.jp:00022532,
 author = {湯浅, 龍彦},
 issue = {3},
 journal = {新潟医学会雑誌, 新潟医学会雑誌},
 month = {Mar},
 note = {In this paper we have summarized our recent studies on brain metabolism by using new experimental techniques, namely in vivo nuclear magnetic resonane spectroscpy (MRS). We have build NMR probes with surface coil, which radiates rf waves and also gets NMR signals from tissues in situ. The NMR machines we have used are JEOL FX27O NMR spectrometer and JEOL GX27O NMR spectrometer, they have a superconducting magnet with 6.35 tesla of magnetic field strength. The measurement conditions we have used are as followed: for ^<31>P-MRS observation frequency (OBSFR); 109.14 MHz, pulse width (PW); 13 μsec, pulse delayed (PD); 2 sec, and FID aquisition times (ACQ); 200 times. The conditions for ^<23>Na-MRS OBSFR 71.35 MHz, PW 15 μsec, PD 0.1 sec, and ACQ 100 times, and for ^<13>C-MRS OBSFR is 67.80 MHz, PW 15 μsec, PD 1.0 sec, ACQ 450 times and gated proton decoupling was done. The post-natal developmental changes of brain phosphorus metabolits were measured by ^<31>P-MRS in rats. The PCr/Pi ratio increased in sigmoid fashon at the time of 2 weeks after birth, and that got plateau at 10 weeks of age. The peakes of phosphomonoesters, highest at birth decreased its intensity during maturation. In the acute brain ischemia a series of events had occured.　The most dramatic events were failure of brain energetics.　We have observed acute failure of energy metabolism in the acute ischemic brain by　^<31>P-MRS. The PCr and ATP of the brain decreased and the Pi peak increased during the brain ischemia induced by bilateral caroted occulusion in the gerbils. Sodium pump function of the brain tissues monitored by ^<23>Na-MRS was failed promptly after ischemia insult, and the function of the pump returned to only partially normal level after reperfusion of the circulation. The glucose metabolites, α-and　β-anomers of glucose, lactate, and glutamate and glutamine were detected by ^<13>C-MRS. The lactate level increased just after BLCO in the gerbil brain. Monitouring of the BBB function is one of fascinating ability of NMR sopectroscopy. We have caluculated Ti relaxation time of the brain tissue by Gd-DTPA enhanced MRI and could find out that hyperpermiability of BBB was continued for a long time after reperfusion of circulation had achieved when the energetics of the brain returned to normal level. In vivo MRS is one of new devises for brain research developing recently in conjunction with widespreading of the methods of MRI.},
 pages = {214--224},
 title = {in vivo NMRスペクトロスコピーによる脳代謝の研究 : 新しい生体計測法としての展望},
 volume = {104},
 year = {1990}
}