@article{oai:niigata-u.repo.nii.ac.jp:00022196,
 author = {成田, 美和子},
 issue = {6},
 journal = {新潟医学会雑誌, 新潟医学会雑誌},
 month = {Jun},
 note = {For the treatment of CML, bone marrow transplantation is thougt to be the only method that consistently restores normal hematopoiesis for long period. However, the indication of allogeneic marrow transplantation is restricted to a small number of patients with CML. As functionally competent Ph^1 negative hematopoietic stem cells are now known to persist in CML, autologous marrow transplantation might be undertaken with curative intent if clonogenic malignant (Ph^1 positive) stem cells could be eliminated from the autograft. For the purpose of developing a effective method to purge Ph^1 positive stem cells, we tried long term CML marrow culture in combination with IFN-α that is clinically established as a drug to inhibit the proliferation of Ph^1 positive cells. The long term culture in the presence of IFN-α (10～10^3 lU/ml) was found to eradicate Ph^1 positive CFU-GM derived colony forming cells in all CML patients in time and dose dependent manner. The effects of IFN-α to the microenvironment and to Ph^1 positive cells are still not so well analysed, but our results suggest that Ph^1 negative CFU-GM are more tolerable than Ph^1 positive ones in long term culture system with IFN-α. Thus, this culture system might be useful method to purging malignant (Ph^1 positive) clone for autologous marrow transplantation in patients with CML.},
 pages = {508--517},
 title = {慢性骨髄性白血病におけるインターフェロン-α添加骨髄長期培養によるPh^1陽性クローンの選択的除去と自家骨髄移植への応用},
 volume = {104},
 year = {1990}
}