@article{oai:niigata-u.repo.nii.ac.jp:00022171, author = {青木, 定夫}, issue = {7}, journal = {新潟医学会雑誌, 新潟医学会雑誌}, month = {Jul}, note = {The surface markers of pathological cells in patients with acute leukemia were analyzed using flow cytometry. In this study, firstly, the phenotype of acute non-lymphatic leukemia (ANLL) cells was determind with reference to the FAB classification. Secondly the two color analysis of acute leukemia cells was performed to detect hybrid acute leukemia (HAL). In 39 cases of ANLL, leukemic blasts of M1 and M2 were positive for CD13, CD33 and anti-HLADR. Those of M3 were positive for CD13 and CD33 but negative for anti-HLADR. In case of M4 and M5 cells, CD14 was positive in addition to these three antibodies. These results showed that the analysis of ANLL cells could related to the FAB classification easily using only a few antibodies. Five of 26 cases of acute leukemia in which two color analyses were performed showed HAL. The clinical diagnosis of 3 of 5 cases was acute lymphoblastic leukemia, that of 1 case was lymphoid crisis of chronic myeloid leukemia, and that of the remaining 1 case was ANLL. The phenotype of leukemic blasts in the former four cases was double positive for CD10 (CALLA), and CD33 and/or CD13. These cases were considered to be biphenotypic leukemia. The leukemic cells in the latter case consisted of two different populations. One population was comprised of CD33 positive cells and the other of CD10 positive cells. This case was considered to be bilineal leukemia. The significance of these HAL cases will be investigated through the accumulation of such cases.}, pages = {537--540}, title = {急性白血病細胞の表面形質の解析: 最近の進歩(第20回新潟造血器腫瘍研究会十周年記念シンポジウム : 造血器腫瘍十年の歩み)}, volume = {104}, year = {1990} }