@article{oai:niigata-u.repo.nii.ac.jp:00002161,
 author = {Toba, Ken and Koike, Tadashi and Hashimoto, Shigeo and Takahashi, Hoyu and Uesugi, Yumiko and Ishikawa, Toru and Aoki, Sadao and Maeo, Syougo and Naito, Makoto and Aizawa, Yoshifusa and Shibata, Akira},
 issue = {10},
 journal = {Leukemia Research, Leukemia Research},
 month = {Oct},
 note = {A patient presented with chronic large granular lymphocytic leukemia after 13 years of mosquito allergy, recurrent fever, hepato-splenomegaly, liver dysfunction and chronic active EBV infection. Surface marker analysis of the leukemic cells showed the presence of CD2, CD8, CD16 and CD56, and absence of CD3, CD57 and HLA-DR. Cell cycle analysis revealed a minimal growth fraction compatible with chronic lymphocytic leukemia, and Southern blots of the cells showed monoclonal integration of EBV. After 5 months of treatment, the patient died from acute transformation of the leukemia and severe liver dysfunction. Cells harvested during chronic phase were analyzed for sensitivity to interleukin-2 (IL-2) and interferon-alpha (IFNα) in vitro by means of surface phenotyping and cell cycle assay. IL-2 induced remarkable growth of the cells, whereas IFNα did not confer a growth advantage. Since IFNα was expected to have no growth induction effect on the leukemia cells, it was administered to the patient to treat the chronic active EBV infection. There is always a risk of conferring a growth advantage upon cancer cells when growth factors or cytokines are administered in vivo as shown in this report.},
 pages = {941--950},
 title = {Characterization and sensitivity to interleukin-2 and interferoin-alpha of leukemic cells from a patient with large granular lymphocytic leukemia associated with chronic active Epstein-Barr virus infection},
 volume = {21},
 year = {1997}
}