{"created":"2021-03-01T06:26:19.688397+00:00","id":21292,"links":{},"metadata":{"_buckets":{"deposit":"d9c792c9-1505-47cf-b139-cb00066b0ecf"},"_deposit":{"id":"21292","owners":[],"pid":{"revision_id":0,"type":"depid","value":"21292"},"status":"published"},"_oai":{"id":"oai:niigata-u.repo.nii.ac.jp:00021292","sets":["453:456","471:537:538:1183"]},"item_7_alternative_title_1":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Degradation of Cerebral Proteins and Effects of Protease Inhibitor in Experimental Cerebral Infarction"}]},"item_7_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1991-08","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"8","bibliographicPageEnd":"553","bibliographicPageStart":"548","bibliographicVolumeNumber":"105","bibliographic_titles":[{"bibliographic_title":"新潟医学会雑誌"},{"bibliographic_title":"新潟医学会雑誌","bibliographic_titleLang":"en"}]}]},"item_7_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Using an immunoblotting technique, I investigated changes in the concentrations of microtubule-associated protein 2 (MAP 2), 200kD neurofilament, tubulin, myelin-associated glycoprotein (MAG) and 2':3'-cyclic nucleotide 3'-phosphodiesterase in the rats brains following occlusion of the left middle cerebral artery or sham operation. Compared with those 4 hours after surgery, concentrations of all proteins decreased significantly in the left hemisphere 3 days after surgery (p<0.01). Microtubule-associated protein 2 was the most susceptible to ischemia. Degradation products of MAP 2 and MAG were detected on the blots. Furthermore, in the contralateral himisphere, concentrations of these two proteins decreased comparing with those in concurrent sham-operated controls 3 days after surgery. Changes in the concentrations of cerebral proteins in the contralateral hemisphere are important for understanding clinical symptoms not attributable solely to the ipsilateral lesion following a focal cerebral stroke. Since MAP 2 is one of the substrate of calpain, E-64c, a synthetic calpain inhibitor, was administered at a dose of 400mg/kg twice a day for 3 days, with the first dose being given before the production of ischemia. The depletion of MAP 2 was significantly inhibited in vivo by E-64c (p<0.05). The results suggest that calpain is partially involved in the degradation of MAP 2, and that the use of calpain inhibitors can be a useful clinical approach to cerebral ischemia.","subitem_description_type":"Abstract"}]},"item_7_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"130358","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Inuzuka, Takashi"}]}]},"item_7_publisher_7":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"新潟医学会"}]},"item_7_select_19":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"publisher"}]},"item_7_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00182415","subitem_source_identifier_type":"NCID"}]},"item_7_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"00290440","subitem_source_identifier_type":"ISSN"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"犬塚, 貴"}],"nameIdentifiers":[{"nameIdentifier":"130357","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-08-16"}],"displaytype":"detail","filename":"105(8)_548-553.pdf","filesize":[{"value":"1.4 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"105(8)_548-553.pdf","url":"https://niigata-u.repo.nii.ac.jp/record/21292/files/105(8)_548-553.pdf"},"version_id":"b519c8eb-dcc7-4e0b-abeb-a4c65eafb2dc"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"focal cerebral ischemia","subitem_subject_scheme":"Other"},{"subitem_subject":"protein","subitem_subject_scheme":"Other"},{"subitem_subject":"calpain E-64c","subitem_subject_scheme":"Other"},{"subitem_subject":"microtubule-associated protein 2","subitem_subject_scheme":"Other"},{"subitem_subject":"局所脳虚血","subitem_subject_scheme":"Other"},{"subitem_subject":"蛋白","subitem_subject_scheme":"Other"},{"subitem_subject":"カルシウム依存性中性プロテアーゼ","subitem_subject_scheme":"Other"},{"subitem_subject":"E-64","subitem_subject_scheme":"Other"},{"subitem_subject":"微小管関連蛋白","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"実験的脳梗塞における脳蛋白質の変化とプロテアーゼインヒビターの効果に関する研究","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"実験的脳梗塞における脳蛋白質の変化とプロテアーゼインヒビターの効果に関する研究"},{"subitem_title":"実験的脳梗塞における脳蛋白質の変化とプロテアーゼインヒビターの効果に関する研究","subitem_title_language":"en"}]},"item_type_id":"7","owner":"1","path":["456","1183"],"pubdate":{"attribute_name":"公開日","attribute_value":"2016-03-10"},"publish_date":"2016-03-10","publish_status":"0","recid":"21292","relation_version_is_last":true,"title":["実験的脳梗塞における脳蛋白質の変化とプロテアーゼインヒビターの効果に関する研究"],"weko_creator_id":"1","weko_shared_id":null},"updated":"2022-12-15T03:51:51.458827+00:00"}