@article{oai:niigata-u.repo.nii.ac.jp:00021292,
 author = {犬塚, 貴},
 issue = {8},
 journal = {新潟医学会雑誌, 新潟医学会雑誌},
 month = {Aug},
 note = {Using an immunoblotting technique, I investigated changes in the concentrations of microtubule-associated protein 2 (MAP 2), 200kD neurofilament, tubulin, myelin-associated glycoprotein (MAG) and 2':3'-cyclic nucleotide 3'-phosphodiesterase in the rats brains following occlusion of the left middle cerebral artery or sham operation. Compared with those 4 hours after surgery, concentrations of all proteins decreased significantly in the left hemisphere 3 days after surgery (p<0.01). Microtubule-associated protein 2 was the most susceptible to ischemia. Degradation products of MAP 2 and MAG were detected on the blots. Furthermore, in the contralateral himisphere, concentrations of these two proteins decreased comparing with those in concurrent sham-operated controls 3 days after surgery. Changes in the concentrations of cerebral proteins in the contralateral hemisphere are important for understanding clinical symptoms not attributable solely to the ipsilateral lesion following a focal cerebral stroke. Since MAP 2 is one of the substrate of calpain, E-64c, a synthetic calpain inhibitor, was administered at a dose of 400mg/kg twice a day for 3 days, with the first dose being given before the production of ischemia. The depletion of MAP 2 was significantly inhibited in vivo by E-64c (p<0.05). The results suggest that calpain is partially involved in the degradation of MAP 2, and that the use of calpain inhibitors can be a useful clinical approach to cerebral ischemia.},
 pages = {548--553},
 title = {実験的脳梗塞における脳蛋白質の変化とプロテアーゼインヒビターの効果に関する研究},
 volume = {105},
 year = {1991}
}