@article{oai:niigata-u.repo.nii.ac.jp:00021176,
 author = {石原, 法子},
 issue = {1},
 journal = {新潟医学会雑誌, 新潟医学会雑誌},
 month = {Jan},
 note = {New cell lines (ECC10 and ECC12) derived from endocrine cell carcinoma (ECC) or small cell carcinoma (SCC) of the stomach were established and characterized. Primary tumor of ECC10 was an early carcinoma composed of ECC with squamous cell carcinoma (SQCC) and of well differentiated tubular adenocarcinoma, while that of ECC12 was an advanced carcinoma solely composed of ECC. Inoculation tumor of ECC10 was hepatic metastatic tumor with ECC and SQCC and that of ECC12 was skin metastatic tumor with ECC. ECC10 grew as both of adherent and floating aggregates and ECC12 grew as adherent aggregates. Both cell lines showed aneuploidy, about 46 hours of population doubling time, and successful xenotransplantation into athymic nude mice. ECC10 and ECC12 were composed of argyrophil cells with constant neurosecretory granules. Immunohistochemically ECC10 was negative for serotonin, peptide hormones, and chromogranin A. But, ECC12 contained some cells positive for serotonin, peptide YY, neuropeptide Y, and chromogranin A. Neuron specific enolase was positive in both cell lines, but carcinoembrionic antigen was negative. ECC10 and ECC12 still retain the characteristics of ECC components of their parent tumors and share many properties with classic type of pulmonary SCC cell lines. They are the first gastric ECC cell lines and can provide unique tools for elucidating the biological properties of gastric ECC.},
 pages = {21--36},
 title = {新しい胃癌株, 胃内分泌細胞癌培養株, の樹立とその特性},
 volume = {106},
 year = {1992}
}