@article{oai:niigata-u.repo.nii.ac.jp:00021139,
 author = {長谷川, 剛},
 issue = {2},
 journal = {新潟医学会雑誌, 新潟医学会雑誌},
 month = {Feb},
 note = {To investigate the erythropoiesis in the human embryo and fetus, the hemopoietic cells in the livers were labeled with EP-1 and CD 34, and were examined by fluorescence-activated cell sorting and immune electron microscopy. The highest percentage of CD34 positive cells was 50% of the all hemopoietic cells in the liver at approximate 35 days of gestational age. Later, the percentage of CD 34 positive cells gradually decreased to 5% by 70 days. The percentage of EP-1 positive (EP-1+) cells increased rapidly from 50% to 80% at approximate 45 days, and later it remained 70% to 85% until 70 days. On Paint-A-Gait analysis, a large number of EP-1+ cells in the liver at 35 days were dominantly present in the fraction of small cells and subsequently scattered in both fractions of small and large cells at 40 days. Finally, most of the EP-1+ cells gathered to the fraction of middle-sized cells. Ultrastructurally, the EP-1+ cells had morphological characteristics of erythroid cells. A small number of both EP-1 and CD34 positive cells, which were regarded as erythroid progenitors, were found throughout all gestational ages. In the livers before 40 days, immature erythroblasts of hepatic origin were not identified, although mature erythroblasts of yolk sac origin and erythroid progenitors were present. Hepatic origin erythroblasts in various differentiation stages were dominant in the livers after 45 days. These findings indicate that the hepatic erythroid progentors, which are derived from hematopoietic stem cell, are present but show no maturation before 40 days of gestational age, were actively differentiated into more mature erythroblasts after approximate 45 days.},
 pages = {124--133},
 title = {ヒト胎芽・胎児肝における造血細胞の分化 : フローサイトメトリーと免疫電顕法を用いて : 第一報 赤芽球系細胞の分化},
 volume = {106},
 year = {1992}
}