@article{oai:niigata-u.repo.nii.ac.jp:00021087, author = {仲澤, 幹雄}, issue = {3}, journal = {新潟医学会雑誌, 新潟医学会雑誌}, month = {Mar}, note = {In the ischemia of isolated perfused hearts, heart ceased its beating within 10min and creatine phosphate (CP) rapidly disappeared. ATP was decreased and disappeared around at 20min of ischemia. Intracellular pH was decreased from 7.2 to 6.0 until 20min of ischemia and sustained this value during the ischemic period. If the heart was reperfused at 15 min after ischemia, CP was recovered completely but, because of the loss of total adenine nucleotide contents, ATP was not. Intracellular Na^+ content was increased during ischemia and it is believed that this Na^+, in turn, increases Ca^<2+> content, a key substanoe of the irreversible damage of the myocardium, upon reperfusion. The course of Na^+ gain during ischemia was not fully understood. The responsibility of Na^+/H^+ exchange to this Na^+ gain has been suggested. Therefore, we have studied that whether or not Na^+/H^+ exchange operates during ischemia using its specific inhibitor, ethylisopropyl amirolide (EIPA). 10^<-5>M of EIPA which almost completely blocked Na^+/H^+ exchange during normoxia had no effect on the ischemia-induced pH change. Thus, we concluded that Na^+/H^+ exchanger did notoperate during ischemia.}, pages = {149--153}, title = {1) 基礎から見た虚血(シンポジウム 虚血心疾患の治療と最近の話題, 第470回新潟医学会)}, volume = {106}, year = {1992} }