@article{oai:niigata-u.repo.nii.ac.jp:00020974,
 author = {村上, 直人},
 issue = {5},
 journal = {新潟医学会雑誌, 新潟医学会雑誌},
 month = {May},
 note = {The cell cycle perturbations induced by ACNU and their role in enhancing effect on radiotherapy were studied using C_6 rat glioma cells. The cell cycle perturbations were analyzed with flow cytometry from 3 to 72 hours after ACNU treatment. The major effect of ACNU on cell cycle progression was G_2M accumulation. Alteration of the DNA histograms after exposure to ACNU (10, 25μg/ml) showed common features ; the G_2M accumulation increased to a maximum at 24hr, remained by 30hr, then decreased gradually. From these analyses, the temporal course of accumulation to G_2M phase of cell cycle in the presence of ACNU (10, 25μg/ml) was demonstrated. To examine whether the G_2M accumulation induced by ACNU is responsible for the potentiation of irradiation, the following study was performed. Cells were irradiated (6 Gy) at various time intervals after ACNU treatment (25ng/ml, 1hr), and post-treatment survival was assessed by colony forming assay. All survival values obtained from the combination treatment schedules were normalized for the ACNU cell kill and then compared with the survival value obtained after irradiation alone. It appeared that combined treatment had a similar synergistic effect in all combination schedules. From these studies, it was concluded that the G_2M accumulation induced by ACNU would not be the partial synchronization as the mechanism responsible for the potentiation of irradiation.},
 pages = {405--412},
 title = {グリオーマに対するACNU・放射線併用療法の基礎的研究 : ACNUの細胞周期内進行に及ぼす効果と放射線の併用時期},
 volume = {106},
 year = {1992}
}