@article{oai:niigata-u.repo.nii.ac.jp:00020973,
 author = {小出, 章},
 issue = {5},
 journal = {新潟医学会雑誌, 新潟医学会雑誌},
 month = {May},
 note = {The mechanism of the endothelial cell damage caused by free AA (arachidonic acid) was investigated in vitro, using purified cerebral microvascular endothelial cells in culture. The endothelial cells were incubated with various concentrations of free AA, 3, 15, 30, 150, 300μM, dissolved in minimal essential medium. The endothelial cell damage was estimated by ^<51>Cr cytotoxicity test and the ability to produce prostacyclin by the endothelial cells after the incubation with free AA. ^<51>Cr release from endothelial cells increased time dependently after the incubation with ≧15μM of free AA, and reached maximum at the end of 24 hours incubation. Pretreatment with dexamethasone, a phospholipase A_2 inhibitor, partially inhibited ^<51>Cr release. However indomethacin, a cyclooxygenase inhibitor and BW755C, a cyclooxygenase and lipoxygenase inhibitor, had no effect on it. Prostacyclin producton by endothelial cells also decreased time dependently after the incubation with ≧30μM of free AA. These findings suggested that free AA itself, not the metabolites, induced the endothelial cell damage. Free AA released from cellular membrane after various cerebral insults may play an important role in occuring the vasogenic cerebral edema.},
 pages = {393--404},
 title = {アラキドン酸による脳微少血管内皮細胞傷害 : 培養細胞を用いた検討},
 volume = {106},
 year = {1992}
}