@article{oai:niigata-u.repo.nii.ac.jp:00020932, author = {杉本, 英夫}, issue = {6}, journal = {新潟医学会雑誌, 新潟医学会雑誌}, month = {Jun}, note = {In order to gain a better understanding of the merits and demerits of sports, the urinalysis of healthy persons before and after running was undertaken. Urine samples were collected from male students before and after 10km running at the maximal speed. The samples were analyzed by measurement of total protein (TP) concentration, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting. The followings were observed; 1) TP concentrations of the control group (before running) were less than 30mg/ dl. 2) TP concentrations elevated following running with their peaks immediately or 1 hour after running, then decreased approximately to the control levels within 3 hours. 3) Total protein excretion within 3 hours was 62~1,089mg [353.1±265.56mg (mean±SD)]. Furthermore, TP excretion of 15(83.3%) of 18 subjects was less than 500mg. 4) SDS-PAGE and Western blotting showed that albumin was the main protein band in the urine which was collected before and 3 hours after running. However, α_2- macroglobulin, immunoglobulin G and transferrin were seen in the peak fraction of TP concentration, in addition to albumin, It is concluded that the runners should decrease the intensity and/or duration of exercises, when one or both of the following changes in urinalysis appeared; 1) Total protein excretion within 3 hours after running was more than 500mg. 2) Other protein band(s) with higher molecular weight than albumin, were seen by SDS-PAGE at higher concentration in the urine collected 3 hours after running than before running, in addition to albumin.}, pages = {503--512}, title = {運動後の尿蛋白成分変化に関するスポーツ医学的研究}, volume = {106}, year = {1992} }