@article{oai:niigata-u.repo.nii.ac.jp:00002033,
 author = {新村, 末雄 and 大輪, 真司},
 issue = {2},
 journal = {Journal of mammalian ova research, Journal of mammalian ova research},
 month = {Apr},
 note = {マンノシダーゼ処置したマウス卵子に媒精して受精率を調べ，卵子表面のマンノースが受精に果たす役割について検討した．0.05，0.1，0.5あるいは1.0単位／ 100 μl の濃度のマンノシダーゼで処置した透明帯除去卵子に媒精すると，受精率は，それぞれ47.8，40.4，28.2および18.8%であり，マンノシダーゼ処置していない対照の卵子の69.2%に比べていずれも有意に低かった．一方，マンノースに親和性のあるレクチンであるCanavalia ensiformis agglutinin（Con A）の結合は，マンノシダーゼ処置していない卵子ではすべてで強度であったが，0.1単位／ 100 μlの濃度のマンノシダーゼで処置した卵子では強度の結合を示したものは44％に減少した．以上の結果から，マンノースは，透明帯とともに細胞膜表面でも，精子が認識するための物質として役割を果たしている可能性が考えられた., Using zona-free mouse eggs treated with mannosidase, the role of mannose on the surface of eggs in fertilization was examined. The fertilization rates of eggs treated with mannosidase at concentrations of 0.05, 0.1, 0.5 or 1.0 units/100 µµl were 47.8, 40.4, 28.2 and 18.8%, respectively, which were all significantly lower than the 69.2% of control eggs with no mannosidase treatment. The binding of Canavalia ensiformis agglutinin (Con A), a lectin with an affinity to mannose, was strong on the surface of all control eggs not treated with mannosidase. However, the rate of eggs showing strong Con A-binding was significantly decreased among those treated with mannosidase at a concentration of 0.1 units/100 µµl (44.0%). These findings suggest that mannose on the surface of the cytoplasmic membrane, in addition to zona pellucida, of eggs has a possible role in sperm recognition.},
 pages = {89--93},
 title = {マンノシダーゼ処置したマウス卵子の受精能},
 volume = {28},
 year = {2011}
}