@article{oai:niigata-u.repo.nii.ac.jp:00020123, author = {竹之内, 辰也}, issue = {6}, journal = {新潟医学会雑誌, 新潟医学会雑誌}, month = {Jun}, note = {A clear cell sarcoma (CCS) cell line, designated as NCS-1, was established in monolayer culture from xenograft line originating from metastatic CCS. Marked karyotypic aberrations and tumorigenicity in nude mice revealed the malignant derivation of the NCS-1 cell line. The cells contained abundant glycogen and were amelanotic by light microscopy. By electron microscopy, however, melanosomes in various developmental stages were seen. Electron microscopic Dopa reaction revealed the presence of tyrosinase activity. By enzyme-linked immunoadsorbent assay, the NCS-1 cells expressed 75-kd glycoprotein which has been identified as a marker of highly differentiated melanoma cells. From these data, the NCS-1 cells were found to retain both cytochemical and morphological properties of CCS. In applying the NCS-1 cells to the panel of melanocytic differentiation, it approximately corresponded to highly differentiated melanoma cells. In conclusion, the present study strongly supported the close cytogenic relationship between CCS and malignant melanoma.}, pages = {533--540}, title = {Clear Cell Sarcoma (Malignant Melanoma of Soft Parts) 由来細胞株の樹立とその性状}, volume = {107}, year = {1993} }