@article{oai:niigata-u.repo.nii.ac.jp:00020117, author = {皆河, 崇志 and 田中, 隆一}, issue = {6}, journal = {新潟医学会雑誌, 新潟医学会雑誌}, month = {Jun}, note = {The biological characteristics of cerebral microvascular endothelial cells (CEs) and the effect of prostagrandins on permeability of CEs were investigated in vitro. Cultured CEs derived from cerebral microvessels were characteirized by the positive reaction for factor-VIII related antigen and the uptake of low density lipoprotein. CEs produced prostacyclin and other prostaglandins, and the ability to produce prostacyclin was unchanged by serial cultivation. CEs aggregated and made capillary-like structures in type 1 collagen. Astrocytes migrated and closely attached to the structures. The cross section of CS co-cultured with astrocytes showed continuous cells surronuding a lumen, and CEs were connected by tight junctions. A model for the blood brain barrier was made using CEs, type 1 collagen and double chambers. Cooling of this system and depletion of the glucose in the media supressed the permeability of CEs for FITC-Albumin. Prostaglandin E_2 and F_<2a>, cyclooxigenase products, had no effect on the permeability of CEs, however Leucotrien C_4, a lipoxygenase product, markedly increased the permeability. Leucotrien C_4 may have an important role in the occurence of the cerebral vasogenic edema.}, pages = {485--490}, title = {3) 脳微小血管内皮細胞の機能と病態(シンポジウム 血管障害の発生メカニズムと予防, 第473回新潟医学会)}, volume = {107}, year = {1993} }