{"created":"2023-07-26T01:13:18.544378+00:00","id":2001075,"links":{},"metadata":{"_buckets":{"deposit":"7623da19-4c8d-4b78-b20d-00b03cc5fdf8"},"_deposit":{"created_by":4,"id":"2001075","owners":[4],"pid":{"revision_id":0,"type":"depid","value":"2001075"},"status":"published"},"_oai":{"id":"oai:niigata-u.repo.nii.ac.jp:02001075","sets":["453:455","471:561:562"]},"author_link":[],"item_6_date_granted_51":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2023-03-23"}]},"item_6_degree_grantor_49":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_language":"ja","subitem_degreegrantor_name":"新潟大学"},{"subitem_degreegrantor_language":"en","subitem_degreegrantor_name":"Niigata University"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"13101","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_6_degree_name_48":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（医学）","subitem_degreename_language":"ja"}]},"item_6_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Enterovirus D68 (EV-D68) causes a range of clinical manifestations, including asthma-like illness, severe respiratory disease, and acute flaccid myelitis. EV-D68 has caused worldwide outbreaks since 2014 and is now recognized as a reemerging infection in many countries. EV-D68-specific PCR assays are widely used for the diagnosis of EV-D68 infection; however, assay sensitivity is a concern because of genetic changes in recently circulated EV-D68. To address this, we summarized EV-D68 sequences from previously reported world outbreaks from 2014 through 2020 on GenBank, and found several mutations at the primer and probe binding sites of the existing EV-D68-specific PCR assays. Subsequently, we designed two novel assays corresponding to the recently reported EV-D68 sequences: an EV-D68-specific real-time and seminested PCR. In an analysis of 22 EV-D68 confirmed cases during a recent EV-D68 outbreak in Japan, the new real-time PCR had higher sensitivity than the existing assay (100% versus 45%, P<0.01) and a lower median CT value (27.8 versus 32.8, P=0.005). Sensitivity was higher for the new nonnested PCR (91%) than for the existing seminested PCR assay (50%, P<0.01). The specificity of the new real-time PCR was 100% using samples from non-EV-D68-infected cases (n=135). In conclusion, our novel assays had higher sensitivity than the existing assay and might lead to more accurate diagnosis of recently circulating EV-D68. To prepare for future EV-D68 outbreaks, EV-D68-specific assays must be continuously monitored and updated.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_6_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"Journal of Clinical Microbiology. 2021, 59(11), e01151-21.","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_6_description_53":{"attribute_name":"学位記番号","attribute_value_mlt":[{"subitem_description":"新大院博(医)第1128号","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_6_dissertation_number_52":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲第5137号"}]},"item_6_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1128/jcm.01151-21","subitem_relation_type_select":"DOI"}}]},"item_6_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2021 American Society for Microbiology. All Rights Reserved.","subitem_rights_language":"en"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Ikuse, Tatsuki","creatorNameLang":"en"},{"creatorName":"幾瀨, 樹","creatorNameLang":"ja"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2023-07-26"}],"displaytype":"detail","filename":"r4nmk1128.pdf","filesize":[{"value":"1.79MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"本文","objectType":"fulltext","url":"https://niigata-u.repo.nii.ac.jp/record/2001075/files/r4nmk1128.pdf"},"version_id":"f63dbb1c-32b2-4b65-aeb3-a1eba459e15c"},{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2023-07-26"}],"displaytype":"detail","filename":"r4nmk1128_a.pdf","filesize":[{"value":"527KB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"要旨","objectType":"abstract","url":"https://niigata-u.repo.nii.ac.jp/record/2001075/files/r4nmk1128_a.pdf"},"version_id":"87e6e35b-793d-4c8f-9416-9470b00d3e5b"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"acute flaccid paralysis","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"enterovirus D68","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"real-time PCR","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"transverse myelitis","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"in silico","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"Development of Novel PCR Assays for Improved Detection of Enterovirus D68","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Development of Novel PCR Assays for Improved Detection of Enterovirus D68","subitem_title_language":"en"},{"subitem_title":"エンテロウイルスD68のよりよい検出のための新規PCRアッセイの開発","subitem_title_language":"ja"}]},"item_type_id":"6","owner":"4","path":["455","562"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2023-07-26"},"publish_date":"2023-07-26","publish_status":"0","recid":"2001075","relation_version_is_last":true,"title":["Development of Novel PCR Assays for Improved Detection of Enterovirus D68"],"weko_creator_id":"4","weko_shared_id":-1},"updated":"2023-07-26T01:13:28.376004+00:00"}