@phdthesis{oai:niigata-u.repo.nii.ac.jp:02000961,
 author = {Shibata, Osamu and 柴田, 理},
 month = {2023-04-27, 2023-04-27},
 note = {This research developed an easy-to-use, reproducible pancreatic cancer animal model utilizing pancreas-targeted hydrodynamic gene delivery to deliver human pancreatic cancer-related genes to the pancreas of wild-type rats. KRASG12D-induced pancreatic intraepithelial neoplasia lesions showed malignant transformation in the main pancreatic duct at 4 weeks and developed acinar-to-ductal metaplasia, which led to pancreatic ductal adenocarcinoma within 5 weeks, and the gene combination of KRASG12D and YAP enhanced these effects. The repeat hydrodynamic gene delivery of KRASG12D + YAP combination at 4 weeks showed acinar-to-ductal metaplasia in all rats and pancreatic ductal adenocarcinoma in 80% of rats 1 week later. Metastatic tumors in the liver, lymph nodes, and subcutaneous lesions and nervous invasion were confirmed. KRASG12D and YAP combined transfer contributes to the E- to N-cadherin switch in pancreatic ductal adenocarcinoma cells and to tumor metastases. This pancreatic cancer model will speed up pancreatic cancer research for novel treatments and biomarkers for early diagnosis., Molecular Therapy Nucleic Acids. 2022, 28, 342-352., 新大院博(医)第1082号},
 school = {新潟大学, Niigata University},
 title = {Establishment of a pancreatic cancer animal model using the pancreas-targeted hydrodynamic gene delivery method},
 year = {}
}