{"created":"2023-03-07T05:13:53.837606+00:00","id":2000836,"links":{},"metadata":{"_buckets":{"deposit":"df43b7af-4911-42ea-8397-bb36454edf86"},"_deposit":{"id":"2000836","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"2000836"},"status":"published"},"_oai":{"id":"oai:niigata-u.repo.nii.ac.jp:02000836","sets":["453:455","471:561:562"]},"author_link":[],"item_6_alternative_title_1":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Role of ATG2B and GSKIP in HSCs","subitem_alternative_title_language":"en"}]},"item_6_date_granted_51":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2022-03-23"}]},"item_6_degree_grantor_49":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_language":"ja","subitem_degreegrantor_name":"新潟大学"},{"subitem_degreegrantor_language":"en","subitem_degreegrantor_name":"Niigata University"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"13101","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_6_degree_name_48":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（医学）","subitem_degreename_language":"ja"}]},"item_6_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"A germline copy number duplication of chromosome 14q32, which contains ATG2B and GSKIP, was identified in families with myeloproliferative neoplasm (MPN). Herein, we show that mice lacking both Atg2b and Gskip, but not either alone, exhibited decreased hematopoiesis, resulting in death in utero accompanied by anemia. In marked contrast to MPN patients with duplication of ATG2B and GSKIP, the number of hematopoietic stem cells (HSCs), in particular long-term HSCs, in double knockout fetal livers were significantly decreased due to increased cell death. Although the remaining HSCs still had the ability to differentiate into hematopoietic progenitor cells, the differentiation efficiency was quite low. Remarkably, mice with knockout of Atg2b or Gskip alone did not show any hematopoietic abnormality. Mechanistically, while loss of both genes had no effect on autophagy, it increased the expression of genes encoding enzymes involved in oxidative phosphorylation. Taken together, our results indicate that Atg2b and Gskip play a synergistic effect in maintaining the pool size of HSCs.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_6_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"Molecular and cellular biology. 2022, 42(1), e0002421.","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_6_description_53":{"attribute_name":"学位記番号","attribute_value_mlt":[{"subitem_description":"新大院博(医)第1050号","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_6_dissertation_number_52":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲第4980号"}]},"item_6_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1128/mcb.00024-21","subitem_relation_type_select":"DOI"}}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Sakai, Shun-suke","creatorNameLang":"en"},{"creatorName":"坂井, 俊介","creatorNameLang":"ja"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2023-03-07"}],"displaytype":"detail","filename":"r3nmk1050.pdf","filesize":[{"value":"1.55MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"本文","objectType":"fulltext","url":"https://niigata-u.repo.nii.ac.jp/record/2000836/files/r3nmk1050.pdf"},"version_id":"be5849a7-7d73-4377-b8f0-fad9c3c3b3d5"},{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2023-03-07"}],"displaytype":"detail","filename":"r3nmk1050_a.pdf","filesize":[{"value":"553KB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"要旨","objectType":"abstract","url":"https://niigata-u.repo.nii.ac.jp/record/2000836/files/r3nmk1050_a.pdf"},"version_id":"ca810235-0652-478a-9e18-d7cce71959c4"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"hematopoietic stem cell","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"hematopoiesis","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"ATG2B","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"GSKIP","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"autophagy","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"myeloproliferative neoplasm","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"Loss of Atg2b and Gskip impairs the maintenance of the hematopoietic stem cell pool size","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Loss of Atg2b and Gskip impairs the maintenance of the hematopoietic stem cell pool size","subitem_title_language":"en"},{"subitem_title":"Atg2bとGskip遺伝子欠損は、造血幹細胞のプールサイズの維持を損なう","subitem_title_language":"ja"}]},"item_type_id":"6","owner":"1","path":["455","562"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2023-03-07"},"publish_date":"2023-03-07","publish_status":"0","recid":"2000836","relation_version_is_last":true,"title":["Loss of Atg2b and Gskip impairs the maintenance of the hematopoietic stem cell pool size"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-03-07T06:31:24.332147+00:00"}