{"created":"2022-07-25T06:27:42.462197+00:00","id":2000642,"links":{},"metadata":{"_buckets":{"deposit":"6462e65d-b948-486e-9f25-9e725d6512f3"},"_deposit":{"id":"2000642","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"2000642"},"status":"published"},"_oai":{"id":"oai:niigata-u.repo.nii.ac.jp:02000642","sets":["432:433:434","453:455"]},"author_link":[],"item_6_date_granted_51":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2022-03-23"}]},"item_6_degree_grantor_49":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_language":"ja","subitem_degreegrantor_name":"新潟大学"},{"subitem_degreegrantor_language":"en","subitem_degreegrantor_name":"Niigata University"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"13101","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_6_degree_name_48":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（歯学）","subitem_degreename_language":"ja"}]},"item_6_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Most oral squamous cell carcinomas (OSCCs) arise from a premalignant lesion, oral epithelial dysplasia; however, useful markers for the early detection of OSCC are lacking. The present study aimed to establish a novel experimental model to observe changes in the sequential expression patterns of mRNAs and proteins in a rat model of tongue cancer using liquid-based cytology techniques. Cytology specimens were collected at 2, 5, 8, 11, 14, 17 and 21 weeks from rats treated with 4-nitroquinoline 1-oxide to induce tongue cancer. The expression of candidate biomarkers was examined by performing immunocytochemistry and reverse transcription-quantitative PCR. The percentage of positively stained nuclei was calculated as the labeling index (LI). All rats developed OSCC of the tongue at 21 weeks. The mRNA expression levels of bromodomain protein 4 (Brd4), c-Myc and Tp53 were upregulated during the progression from negative for intraepithelial lesion or malignancy to squamous cell carcinoma (SCC). Brd4- and c-Myc-LI increased in low-grade squamous intraepithelial lesion, high-grade squamous intraepithelial lesion and SCC specimens. p53-LI was significantly increased in SCC specimens. This novel experimental model allowed the observation of sequential morphological changes and the expression patterns of mRNAs and proteins during carcinogenesis. Combining immunocytochemistry with cytology-based diagnoses may potentially improve the diagnostic accuracy of OSCC.","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_6_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"Oncology Letters. 2022, 23(3), 76.","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_6_description_53":{"attribute_name":"学位記番号","attribute_value_mlt":[{"subitem_description":"新大院博(歯)第512号","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_6_dissertation_number_52":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲第5026号"}]},"item_6_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.3892/ol.2022.13196","subitem_relation_type_select":"DOI"}}]},"item_6_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International","subitem_rights_language":"en","subitem_rights_resource":"https://creativecommons.org/licenses/by-nc-nd/4.0/"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Kawaharada, Masami","creatorNameLang":"en"},{"creatorName":"河原田, 壮史","creatorNameLang":"ja"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"date":[{"dateType":"Available","dateValue":"2022-07-25"}],"displaytype":"detail","filename":"r3ndk512.pdf","filesize":[{"value":"1.67MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"本文","objectType":"fulltext","url":"https://niigata-u.repo.nii.ac.jp/record/2000642/files/r3ndk512.pdf"},"version_id":"20432db0-c827-4ae7-acf2-1c18a9303b33"},{"date":[{"dateType":"Available","dateValue":"2022-07-25"}],"displaytype":"detail","filename":"r3ndk512_a.pdf","filesize":[{"value":"363KB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"要旨","objectType":"abstract","url":"https://niigata-u.repo.nii.ac.jp/record/2000642/files/r3ndk512_a.pdf"},"version_id":"02de7077-fddb-4912-86bf-6177c5a85cb4"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"oral squamous cell carcinoma","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"oral cancer","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"bromodomain protein 4","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"c-Myc","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"p53","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"cytology","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"liquid-based cytology","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"immunocytochemistry","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"4-nitroquinoline 1-oxide","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"Novel cytological model for the identification of early oral cancer diagnostic markers : The carcinoma sequence model","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Novel cytological model for the identification of early oral cancer diagnostic markers : The carcinoma sequence model","subitem_title_language":"en"},{"subitem_title":"口腔がん早期診断用マーカーを同定するための新規発がんモデルの確立","subitem_title_language":"ja"}]},"item_type_id":"6","owner":"1","path":["455","434"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2022-07-25"},"publish_date":"2022-07-25","publish_status":"0","recid":"2000642","relation_version_is_last":true,"title":["Novel cytological model for the identification of early oral cancer diagnostic markers : The carcinoma sequence model"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2022-12-15T04:18:02.646556+00:00"}