{"created":"2022-05-27T06:27:38.585630+00:00","id":2000535,"links":{},"metadata":{"_buckets":{"deposit":"f0a66339-cf58-4f87-ac97-1d3880287a16"},"_deposit":{"id":"2000535","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"2000535"},"status":"published"},"_oai":{"id":"oai:niigata-u.repo.nii.ac.jp:02000535","sets":["432:1291:1292:1653632400081","453:456"]},"author_link":[],"item_1627361970403":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_7_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2015-12","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"66","bibliographicPageStart":"59","bibliographicVolumeNumber":"45","bibliographic_titles":[{"bibliographic_title":"新潟歯学会雑誌","bibliographic_titleLang":"ja"}]}]},"item_7_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Periodontitis is a biofilm-induced chronic inflammatory disease that causes gingival inflammation and attachment loss. Destruction of periodontal tissue is a consequence of host immune inflammatory responses induced by periodontal microorganisms. Wnt5a is strongly associated with inflammatory responses and shown by several studies to be involved in inflammatory diseases including periodontitis. Soluble frizzled related protein-5 (sFRP5) is a homolog of Wnt5a receptor, frizzled protein. sFRP5 is a known inhibitor of Wnt5a signaling. To investigate the correlation of Wnt5a and sFRP5 in periodontitis and healthy tissues, experiments were set up using both clinical specimens and further investigation with stimulations of human gingival epithelial cells (HGECs). Wnt5a and sFRP5 mRNA expressions and IL-8 mRNA and chemokine levels in HGECs were determined by quantitative real-time PCR and enzyme-linked immunosorbent assay, respectively. Results showed higher level of Wnt5a expression in periodontitis group than in healthy group and vice versa for sFRP5 expression. Lipopolysaccharide from Porphyromonas gingivalis or Escherichia coli induced Wnt5a expression and reduced sFRP5 expression. Moreover, sFRP5 mediated anti-inflammatory effects by inhibiting interleukin-8 production in HGECs. In conclusion, inverse correlation of Wnt5a and sFRP5 expression was shown for the first time and human gingival epithelial cells were also proved to express both Wnt5a and sFRP5. Wnt5a appears to an important target for intervention in periodontitis and sFRP5 is likely a promising therapeutic compound.","subitem_description_language":"en","subitem_description_type":"Abstract"},{"subitem_description":"歯周病は細菌のバイオフィルムによって引き起こされる感染症であり，宿主との免疫反応によって歯周組織の破壊と歯槽骨の吸収を引き起こす．Wnt5aは，歯周病を含む炎症反応と強い関連が報告されている．Soluble frizzled related protein-5(sFRP5)はWnt5aの受容体の相同体であり，Wnt5a経路を阻害する．歯周炎組織と健常歯肉組織においてWnt5aとsFRP5の関連性については不明な点が多い．そこで本研究では，ヒト歯肉サンプルでのWnt5aとsFRP5のmRNA発現，ヒト歯肉上皮細胞(HGECs)におけるinterleukin-8(IL-8)mRNAとケモカインのレベルを定量的PCRおよびELISAにて各々解析した．健常組織と比べて歯周病罹患組織においてWnt5a遺伝子発現は高く，sFRP5発現は抑制されていることが明らかになった．同様に，HGECsにおいてもPorphyromonas gingivalisリポ多糖によりWnt5a発現の上昇とsFRP5発現の抑制が認められた．加えて，sFRP5のHGECsにおけるIL-8の産生抑制効果が認められた．以上の事から，HGECsではWnt5aとsFRP5は双方発現し負の相関関係にあることが初めて示された。また，Wnt5aは歯周病治療の対象となること，sFPR5が歯周病治療化合物として使用可能であることが示唆された．","subitem_description_language":"ja","subitem_description_type":"Abstract"}]},"item_7_publisher_7":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"新潟歯学会","subitem_publisher_language":"ja"}]},"item_7_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00182415","subitem_source_identifier_type":"NCID"}]},"item_7_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0385-0153","subitem_source_identifier_type":"PISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"前川, 知樹","creatorNameLang":"ja"},{"creatorName":"Maekawa, Tomoki","creatorNameLang":"en"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2022-05-27"}],"displaytype":"detail","filename":"45(2)_59-66.pdf","filesize":[{"value":"225KB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://niigata-u.repo.nii.ac.jp/record/2000535/files/45(2)_59-66.pdf"},"version_id":"c22d46cc-2303-4aa8-af57-6f8340a2595b"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"歯肉上皮細胞","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"炎症","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"歯周病","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"Gingival epithelial cells","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"inflammation","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"periodontitis","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"歯肉組織におけるWnt5aとsFRP5の発現機能解析","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"歯肉組織におけるWnt5aとsFRP5の発現機能解析","subitem_title_language":"ja"},{"subitem_title":"Investigation of Wnt5a and sFRP5 Expressions in Healthy and Chronic Periodontitis Tissues","subitem_title_language":"en"}]},"item_type_id":"7","owner":"1","path":["456","1653632400081"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2022-05-27"},"publish_date":"2022-05-27","publish_status":"0","recid":"2000535","relation_version_is_last":true,"title":["歯肉組織におけるWnt5aとsFRP5の発現機能解析"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2022-12-15T04:35:58.436861+00:00"}