{"created":"2022-05-10T05:52:33.931052+00:00","id":2000459,"links":{},"metadata":{"_buckets":{"deposit":"623c10ff-8b58-48e1-be35-52818df6351f"},"_deposit":{"id":"2000459","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"2000459"},"status":"published"},"_oai":{"id":"oai:niigata-u.repo.nii.ac.jp:02000459","sets":["453:455","471:561:562"]},"author_link":[],"control_number":"2000459","item_6_date_granted_51":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2021-09-21"}]},"item_6_degree_grantor_49":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_language":"ja","subitem_degreegrantor_name":"新潟大学"},{"subitem_degreegrantor_language":"en","subitem_degreegrantor_name":"Niigata University"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"13101","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_6_degree_name_48":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（医学）","subitem_degreename_language":"ja"}]},"item_6_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"KRAS is the most frequently mutated in ovarian endometriosis. However, it is unclear whether the KRAS mutant allele's mRNA is expressed and plays a biological role in ovarian endometriosis. Here, we performed mutation-specific RNA in situ hybridization to evaluate mutant allele expression of KRAS p.G12V, the most frequently detected mutation in ovarian endometriosis in our previous study, in formalin-fixed paraffin-embedded tissue (FFPE) samples of ovarian endometriosis, cancer cell lines, and ovarian cancers. First, we verified that mutant or wild-type allele of KRAS were expressed in all 5 cancer cell lines and 9 ovarian cancer cases corresponding to the mutation status. Next, we applied this assay to 26 ovarian endometriosis cases, and observed mutant allele expression of KRAS p.G12V in 10 cases. Mutant or wild-type allele of KRAS were expressed in line with mutation status in 12 available endometriosis cases for which KRAS gene sequence was determined. Comparison of clinical features between ovarian endometriosis with KRAS p.G12V mutant allele expression and with KRAS wild-type showed that KRAS p.G12V mutant allele expression was significantly associated with inflammation in ovarian endometriosis. Finally, we assessed the spatial distribution of KRAS mutant allele expression in 5 endometriosis cases by performing multiregional sampling. Intratumor heterogeneity of KRAS mutant allele expression was observed in two endometriosis cases, whereas the spatial distribution of KRAS p.G12V mutation signals were diffuse and homogenous in ovarian cancer. In conclusion, evaluation of oncogene mutant expression will be useful for clarifying the biological significance of oncogene mutations in benign tumors.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_6_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"Cancer science. 2021, 112(5), 2020-2032.","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_6_description_53":{"attribute_name":"学位記番号","attribute_value_mlt":[{"subitem_description":"新大院博(医)第1017号","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_6_dissertation_number_52":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲第4927号"}]},"item_6_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1111/cas.14871","subitem_relation_type_select":"DOI"}}]},"item_6_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"【○!C】 2021 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association.","subitem_rights_language":"en"},{"subitem_rights":"Creative Commons Attribution-NonCommercial 4.0 International","subitem_rights_language":"en","subitem_rights_resource":"https://creativecommons.org/licenses/by-nc/4.0/"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Yachida, Nozomi","creatorNameLang":"en"},{"creatorName":"谷地田, 希","creatorNameLang":"ja"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"date":[{"dateType":"Available","dateValue":"2022-05-10"}],"displaytype":"detail","filename":"r3nmk1017.pdf","filesize":[{"value":"1.51MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"本文","objectType":"fulltext","url":"https://niigata-u.repo.nii.ac.jp/record/2000459/files/r3nmk1017.pdf"},"version_id":"2547bcc7-167a-4292-9f7a-5e1099d86b6f"},{"date":[{"dateType":"Available","dateValue":"2022-05-10"}],"displaytype":"detail","filename":"r3nmk1017_a.pdf","filesize":[{"value":"442KB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"要旨","objectType":"abstract","url":"https://niigata-u.repo.nii.ac.jp/record/2000459/files/r3nmk1017_a.pdf"},"version_id":"fa43d78c-f01b-441f-974e-71e76841b3d4"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"endometriosis","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"in situ hybridization","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"intratumor heterogeneity","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"KRAS","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"mutant allele expression","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"Biological significance of KRAS mutant allele expression in ovarian endometriosis","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Biological significance of KRAS mutant allele expression in ovarian endometriosis","subitem_title_language":"en"},{"subitem_title":"卵巣子宮内膜症におけるKRAS変異アリル発現の生物学的意義","subitem_title_language":"ja"}]},"item_type_id":"6","owner":"1","path":["455","562"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2022-05-10"},"publish_date":"2022-05-10","publish_status":"0","recid":"2000459","relation_version_is_last":true,"title":["Biological significance of KRAS mutant allele expression in ovarian endometriosis"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2022-12-15T04:16:51.877989+00:00"}