@phdthesis{oai:niigata-u.repo.nii.ac.jp:02000443,
 author = {Makishi, Sanako and 真喜志, 佐奈子},
 month = {2022-05-09, 2022-05-09},
 note = {After dental implantation, osteopontin (OPN) is deposited on the hydroxyapatite (HA) blasted implant surface followed by direct osteogenesis, which is significantly disturbed in Opn-knockout (KO) mice. However, whether applying OPN on the implant surface promotes direct osteogenesis remains unclarified. This study analyzed the effects of various OPN modified protein/peptides coating on the healing patterns of the bone-implant interface after immediately placed implantation in the maxilla of four-week-old Opn-KO and wild-type (WT) mice. The decalcified samples were processed for immunohistochemistry for OPN and Ki67 and TRAP histochemistry. In the WT mice, the proliferative activity in the HA binding peptide-OPN mimic peptide fusion coated group was significantly higher than that in the control group from day 3 to week 1, and OPN deposition on the implant surface significantly increased in the recombinant-mouse-OPN (rOPN) group compared to the RGDS group on week 2. The rOPN group achieved the same rates of direct osteogenesis and osseointegration as those in the control group in a half period (on week 2). None of the implant surfaces could rescue the direct osteogenesis in the healing process in the Opn-KO mice. These results suggest that the rOPN coated implant enhances direct osteogenesis during osseointegration following implantation., International Journal of Molecular Sciences. 2022, 23(3), 1039., 新大院博(歯)第494号},
 school = {新潟大学, Niigata University},
 title = {Osteopontin on the dental implant surface promotes direct osteogenesis in osseointegration},
 year = {}
}