{"created":"2022-04-15T06:23:55.520165+00:00","id":2000371,"links":{},"metadata":{"_buckets":{"deposit":"08a0a84c-2761-49c4-9d94-03498f634d19"},"_deposit":{"id":"2000371","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"2000371"},"status":"published"},"_oai":{"id":"oai:niigata-u.repo.nii.ac.jp:02000371","sets":["432:433:434","453:455"]},"author_link":[],"item_6_date_granted_51":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2021-03-23"}]},"item_6_degree_grantor_49":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_language":"ja","subitem_degreegrantor_name":"新潟大学"},{"subitem_degreegrantor_language":"en","subitem_degreegrantor_name":"Niigata University"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"13101","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_6_degree_name_48":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（歯学）","subitem_degreename_language":"ja"}]},"item_6_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Objective: Rice peptide has antibacterial properties that have been tested in planktonic bacterial culture. However, bacteria form biofilm at disease sites and are resistant to antibacterial agents. The aim of this study was to clarify the mechanisms of action of rice peptide and its amino acid substitution against periodontopathic bacteria and their antibiofilm effects. Design: Porphyromonas gingivalis and Fusobacterium nucleatum were treated with AmyI-1-18 rice peptide or its arginine-substituted analog, G12R, under anaerobic conditions. The amount of biofilm was evaluated by crystal violet staining. The integrity of the bacteria cytoplasmic membrane was studied in a propidium iodide (PI) stain assay and transmission electron microscopy (TEM). Results: Both AmyI-1-18 and G12R inhibited biofilm formation of P. gingivalis and F. nucleatum; in particular, G12R inhibited F. nucleatum at lower concentrations. However, neither peptide eradicated established biofilms significantly. According to the minimum inhibitory concentration and minimum bactericidal concentration against P. gingivalis, AmyI-1-18 has bacteriostatic properties and G12R has bactericidal activity, and both peptides showed bactericidal activity against F. nucleatum. PI staining and TEM analysis indicated that membrane disruption by G12R was enhanced, which suggests that the replacement amino acid reinforced the electostatic interaction between the peptide and bacteria by increase of cationic charge and α-helix content. Conclusions: Rice peptide inhibited biofilm formation of P. gingivalis and F. nucleatum, and bactericidal activity via membrane destruction was enhanced by amino acid substitution.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_6_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"Archives of Oral Biology. 2021, 121, 104956.","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_6_description_53":{"attribute_name":"学位記番号","attribute_value_mlt":[{"subitem_description":"新大院博(歯)第483号","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_6_dissertation_number_52":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲第4875号"}]},"item_6_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1016/j.archoralbio.2020.104956","subitem_relation_type_select":"DOI"}}]},"item_6_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"【○!C】 2020 Elsevier Ltd. All rights reserved.","subitem_rights_language":"en"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Matsugishi, Aoi","creatorNameLang":"en"},{"creatorName":"松岸, 葵","creatorNameLang":"ja"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"date":[{"dateType":"Available","dateValue":"2022-04-15"}],"displaytype":"detail","filename":"r2ndk483.pdf","filesize":[{"value":"4.33MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"本文","objectType":"fulltext","url":"https://niigata-u.repo.nii.ac.jp/record/2000371/files/r2ndk483.pdf"},"version_id":"8764f9d7-1fae-4094-818c-4c781e865a89"},{"date":[{"dateType":"Available","dateValue":"2022-04-15"}],"displaytype":"detail","filename":"r2ndk483_a.pdf","filesize":[{"value":"239KB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"要旨","objectType":"abstract","url":"https://niigata-u.repo.nii.ac.jp/record/2000371/files/r2ndk483_a.pdf"},"version_id":"6f511e27-57b5-4a7d-a5c0-f24eb0bf4d87"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Antibacterial peptide","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Amino acid substitution","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Biofilms","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Porphyromonas gignivalis","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Fusobacterium nucleatum","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"Rice peptide with amino acid substitution inhibits biofilm formation by Porphyromonas gingivalis and Fusobacterium nucleatum","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Rice peptide with amino acid substitution inhibits biofilm formation by Porphyromonas gingivalis and Fusobacterium nucleatum","subitem_title_language":"en"},{"subitem_title":"コメ由来ペプチドのアミノ酸置換体はPorphyromonas gingivalisとFusobacterium nucleatumのバイオフィルム形成を阻害する","subitem_title_language":"ja"}]},"item_type_id":"6","owner":"1","path":["455","434"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2022-04-15"},"publish_date":"2022-04-15","publish_status":"0","recid":"2000371","relation_version_is_last":true,"title":["Rice peptide with amino acid substitution inhibits biofilm formation by Porphyromonas gingivalis and Fusobacterium nucleatum"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2022-12-15T04:13:43.166446+00:00"}