{"_buckets": {"deposit": "0e4097a9-a4dc-4008-9579-858a003bb03c"}, "_deposit": {"id": "2000370", "owners": [1], "pid": {"revision_id": 0, "type": "depid", "value": "2000370"}, "status": "published"}, "_oai": {"id": "oai:niigata-u.repo.nii.ac.jp:02000370", "sets": ["455", "434"]}, "author_link": [], "control_number": "2000370", "item_6_date_granted_51": {"attribute_name": "学位授与年月日", "attribute_value_mlt": [{"subitem_dategranted": "2021-03-23"}]}, "item_6_degree_grantor_49": {"attribute_name": "学位授与機関", "attribute_value_mlt": [{"subitem_degreegrantor": [{"subitem_degreegrantor_language": "ja", "subitem_degreegrantor_name": "新潟大学"}, {"subitem_degreegrantor_language": "en", "subitem_degreegrantor_name": "Niigata University"}], "subitem_degreegrantor_identifier": [{"subitem_degreegrantor_identifier_name": "13101", "subitem_degreegrantor_identifier_scheme": "kakenhi"}]}]}, "item_6_degree_name_48": {"attribute_name": "学位名", "attribute_value_mlt": [{"subitem_degreename": "博士（歯学）", "subitem_degreename_language": "ja"}]}, "item_6_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "Image-based cell/colony analyses offer promising solutions to compensate for the lack of quality control (QC) tools for noninvasive monitoring of cultured cells, a regulatory challenge in regenerative medicine. Here, the feasibility of two image analysis algorithms, optical flow and normalised cross-correlation, to noninvasively measure cell/colony motion in human primary oral keratinocytes for screening the proliferative capacity of cells in the early phases of cell culture were examined. We applied our software to movies converted from 96 consecutive time-lapse phase-contrast images of an oral keratinocyte culture. After segmenting the growing colonies, two indices were calculated based on each algorithm. The correlation between each index of the colonies and their proliferative capacity was evaluated. The software was able to assess cell/colony motion noninvasively, and each index reflected the observed cell kinetics. A positive linear correlation was found between cell/colony motion and proliferative capacity, indicating that both algorithms are potential tools for QC.", "subitem_description_language": "en", "subitem_description_type": "Abstract"}]}, "item_6_description_5": {"attribute_name": "内容記述", "attribute_value_mlt": [{"subitem_description": "Journal of Tissue Engineering, 2019, 10, 1-12.", "subitem_description_language": "en", "subitem_description_type": "Other"}]}, "item_6_description_53": {"attribute_name": "学位記番号", "attribute_value_mlt": [{"subitem_description": "新大院博(歯)第482号", "subitem_description_language": "ja", "subitem_description_type": "Other"}]}, "item_6_dissertation_number_52": {"attribute_name": "学位授与番号", "attribute_value_mlt": [{"subitem_dissertationnumber": "甲第4874号"}]}, "item_6_relation_14": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "https://doi.org/10.1177/2041731419881528", "subitem_relation_type_select": "DOI"}}]}, "item_6_rights_15": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "【○!C】 The Author(s) 2019", "subitem_rights_language": "en"}, {"subitem_rights": "Creative Commons Attribution-NonCommercial 4.0 International", "subitem_rights_language": "en", "subitem_rights_resource": "https://creativecommons.org/licenses/by-nc/4.0/"}]}, "item_access_right": {"attribute_name": "アクセス権", "attribute_value_mlt": [{"subitem_access_right": "open access", "subitem_access_right_uri": "http://purl.org/coar/access_right/c_abf2"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Hoshikawa, Emi", "creatorNameLang": "en"}, {"creatorName": "干川, 絵美", "creatorNameLang": "ja"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"date": [{"dateType": "Available", "dateValue": "2022-04-15"}], "displaytype": "detail", 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"item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "Oral keratinocyte", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "quality control", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "regenerative medicine", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "cell/colony motion", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "image analysis", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "doctoral thesis", "resourceuri": "http://purl.org/coar/resource_type/c_db06"}]}, "item_title": "Noninvasive measurement of cell/colony motion using image analysis methods to evaluate the proliferative capacity of oral keratinocytes as a tool for quality control in regenerative medicine", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Noninvasive measurement of cell/colony motion using image analysis methods to evaluate the proliferative capacity of oral keratinocytes as a tool for quality control in regenerative medicine", "subitem_title_language": "en"}, {"subitem_title": "口腔ケラチノサイトの増殖能を評価するための画像解析法を用いた細胞/コロニー運動の非侵襲的測定 : 再生医療用細胞品質管理ツールとして", "subitem_title_language": "ja"}]}, "item_type_id": "6", "owner": "1", "path": ["455", "434"], "permalink_uri": "http://hdl.handle.net/10191/0002000370", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2022-04-15"}, "publish_date": "2022-04-15", "publish_status": "0", "recid": "2000370", "relation": {}, "relation_version_is_last": true, "title": ["Noninvasive measurement of cell/colony motion using image analysis methods to evaluate the proliferative capacity of oral keratinocytes as a tool for quality control in regenerative medicine"], "weko_shared_id": -1}