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口腔ケラチノサイトの増殖能を評価するための画像解析法を用いた細胞/コロニー運動の非侵襲的測定 : 再生医療用細胞品質管理ツールとして
http://hdl.handle.net/10191/0002000370
http://hdl.handle.net/10191/00020003707c19380f-12ac-4c86-a559-f5665d6251a4
名前 / ファイル | ライセンス | アクション |
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本文 (1.19MB)
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要旨 (239KB)
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Item type | 学位論文 / Thesis or Dissertation(1) | |||||||||
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公開日 | 2022-04-15 | |||||||||
タイトル | ||||||||||
言語 | en | |||||||||
タイトル | Noninvasive measurement of cell/colony motion using image analysis methods to evaluate the proliferative capacity of oral keratinocytes as a tool for quality control in regenerative medicine | |||||||||
タイトル | ||||||||||
言語 | ja | |||||||||
タイトル | 口腔ケラチノサイトの増殖能を評価するための画像解析法を用いた細胞/コロニー運動の非侵襲的測定 : 再生医療用細胞品質管理ツールとして | |||||||||
言語 | ||||||||||
言語 | eng | |||||||||
キーワード | ||||||||||
言語 | en | |||||||||
主題Scheme | Other | |||||||||
主題 | Oral keratinocyte | |||||||||
キーワード | ||||||||||
言語 | en | |||||||||
主題Scheme | Other | |||||||||
主題 | quality control | |||||||||
キーワード | ||||||||||
言語 | en | |||||||||
主題Scheme | Other | |||||||||
主題 | regenerative medicine | |||||||||
キーワード | ||||||||||
言語 | en | |||||||||
主題Scheme | Other | |||||||||
主題 | cell/colony motion | |||||||||
キーワード | ||||||||||
言語 | en | |||||||||
主題Scheme | Other | |||||||||
主題 | image analysis | |||||||||
資源タイプ | ||||||||||
資源 | http://purl.org/coar/resource_type/c_db06 | |||||||||
タイプ | doctoral thesis | |||||||||
アクセス権 | ||||||||||
アクセス権 | open access | |||||||||
アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||||||
著者 |
干川, 絵美
× 干川, 絵美
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抄録 | ||||||||||
内容記述タイプ | Abstract | |||||||||
内容記述 | Image-based cell/colony analyses offer promising solutions to compensate for the lack of quality control (QC) tools for noninvasive monitoring of cultured cells, a regulatory challenge in regenerative medicine. Here, the feasibility of two image analysis algorithms, optical flow and normalised cross-correlation, to noninvasively measure cell/colony motion in human primary oral keratinocytes for screening the proliferative capacity of cells in the early phases of cell culture were examined. We applied our software to movies converted from 96 consecutive time-lapse phase-contrast images of an oral keratinocyte culture. After segmenting the growing colonies, two indices were calculated based on each algorithm. The correlation between each index of the colonies and their proliferative capacity was evaluated. The software was able to assess cell/colony motion noninvasively, and each index reflected the observed cell kinetics. A positive linear correlation was found between cell/colony motion and proliferative capacity, indicating that both algorithms are potential tools for QC. | |||||||||
言語 | en | |||||||||
内容記述 | ||||||||||
内容記述タイプ | Other | |||||||||
内容記述 | Journal of Tissue Engineering, 2019, 10, 1-12. | |||||||||
言語 | en | |||||||||
DOI | ||||||||||
識別子タイプ | DOI | |||||||||
関連識別子 | https://doi.org/10.1177/2041731419881528 | |||||||||
権利 | ||||||||||
言語 | en | |||||||||
権利情報 | 【○!C】 The Author(s) 2019 | |||||||||
権利 | ||||||||||
言語 | en | |||||||||
権利情報Resource | https://creativecommons.org/licenses/by-nc/4.0/ | |||||||||
権利情報 | Creative Commons Attribution-NonCommercial 4.0 International | |||||||||
学位名 | ||||||||||
言語 | ja | |||||||||
学位名 | 博士(歯学) | |||||||||
学位授与機関 | ||||||||||
学位授与機関識別子Scheme | kakenhi | |||||||||
学位授与機関識別子 | 13101 | |||||||||
言語 | ja | |||||||||
学位授与機関名 | 新潟大学 | |||||||||
言語 | en | |||||||||
学位授与機関名 | Niigata University | |||||||||
学位授与年月日 | ||||||||||
学位授与年月日 | 2021-03-23 | |||||||||
学位授与番号 | ||||||||||
学位授与番号 | 甲第4874号 | |||||||||
学位記番号 | ||||||||||
内容記述タイプ | Other | |||||||||
内容記述 | 新大院博(歯)第482号 | |||||||||
言語 | ja |