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  1. 260 大学院医歯学総合研究科(歯)
  2. 60 博士学位論文
  3. 10 博士学位論文
  1. 0 資料タイプ別
  2. 02 学位論文

口腔ケラチノサイトの増殖能を評価するための画像解析法を用いた細胞/コロニー運動の非侵襲的測定 : 再生医療用細胞品質管理ツールとして

http://hdl.handle.net/10191/0002000370
http://hdl.handle.net/10191/0002000370
7c19380f-12ac-4c86-a559-f5665d6251a4
名前 / ファイル ライセンス アクション
r2ndk482.pdf 本文 (1.19MB)
r2ndk482_a.pdf 要旨 (239KB)
Item type 学位論文 / Thesis or Dissertation(1)
公開日 2022-04-15
タイトル
言語 en
タイトル Noninvasive measurement of cell/colony motion using image analysis methods to evaluate the proliferative capacity of oral keratinocytes as a tool for quality control in regenerative medicine
タイトル
言語 ja
タイトル 口腔ケラチノサイトの増殖能を評価するための画像解析法を用いた細胞/コロニー運動の非侵襲的測定 : 再生医療用細胞品質管理ツールとして
言語
言語 eng
キーワード
言語 en
主題Scheme Other
主題 Oral keratinocyte
キーワード
言語 en
主題Scheme Other
主題 quality control
キーワード
言語 en
主題Scheme Other
主題 regenerative medicine
キーワード
言語 en
主題Scheme Other
主題 cell/colony motion
キーワード
言語 en
主題Scheme Other
主題 image analysis
資源タイプ
資源 http://purl.org/coar/resource_type/c_db06
タイプ doctoral thesis
アクセス権
アクセス権 open access
アクセス権URI http://purl.org/coar/access_right/c_abf2
著者 干川, 絵美

× 干川, 絵美

en Hoshikawa, Emi

ja 干川, 絵美

Search repository
抄録
内容記述タイプ Abstract
内容記述 Image-based cell/colony analyses offer promising solutions to compensate for the lack of quality control (QC) tools for noninvasive monitoring of cultured cells, a regulatory challenge in regenerative medicine. Here, the feasibility of two image analysis algorithms, optical flow and normalised cross-correlation, to noninvasively measure cell/colony motion in human primary oral keratinocytes for screening the proliferative capacity of cells in the early phases of cell culture were examined. We applied our software to movies converted from 96 consecutive time-lapse phase-contrast images of an oral keratinocyte culture. After segmenting the growing colonies, two indices were calculated based on each algorithm. The correlation between each index of the colonies and their proliferative capacity was evaluated. The software was able to assess cell/colony motion noninvasively, and each index reflected the observed cell kinetics. A positive linear correlation was found between cell/colony motion and proliferative capacity, indicating that both algorithms are potential tools for QC.
言語 en
内容記述
内容記述タイプ Other
内容記述 Journal of Tissue Engineering, 2019, 10, 1-12.
言語 en
DOI
識別子タイプ DOI
関連識別子 https://doi.org/10.1177/2041731419881528
権利
言語 en
権利情報 【○!C】 The Author(s) 2019
権利
言語 en
権利情報Resource https://creativecommons.org/licenses/by-nc/4.0/
権利情報 Creative Commons Attribution-NonCommercial 4.0 International
学位名
言語 ja
学位名 博士(歯学)
学位授与機関
学位授与機関識別子Scheme kakenhi
学位授与機関識別子 13101
言語 ja
学位授与機関名 新潟大学
言語 en
学位授与機関名 Niigata University
学位授与年月日
学位授与年月日 2021-03-23
学位授与番号
学位授与番号 甲第4874号
学位記番号
内容記述タイプ Other
内容記述 新大院博(歯)第482号
言語 ja
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