{"created":"2022-04-15T06:23:49.270516+00:00","id":2000369,"links":{},"metadata":{"_buckets":{"deposit":"5b88a07e-308c-44de-b6ec-2f244893d701"},"_deposit":{"id":"2000369","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"2000369"},"status":"published"},"_oai":{"id":"oai:niigata-u.repo.nii.ac.jp:02000369","sets":["432:433:434","453:455"]},"author_link":[],"item_6_date_granted_51":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2021-03-23"}]},"item_6_degree_grantor_49":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_language":"ja","subitem_degreegrantor_name":"新潟大学"},{"subitem_degreegrantor_language":"en","subitem_degreegrantor_name":"Niigata University"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"13101","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_6_degree_name_48":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（歯学）","subitem_degreename_language":"ja"}]},"item_6_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Aggregatibacter actinomycetemcomitans is considered to be associated with periodontitis. Leukotoxin (LtxA), which destroys leukocytes in humans, is one of this bacterium's major virulence factors. Amounts of neutrophil elastase (NE), which is normally localized in the cytoplasm of neutrophils, are reportedly increased in the saliva of patients with periodontitis. However, the mechanism by which NE is released from human neutrophils and the role of NE in periodontitis is unclear. In the present study, it was hypothesized that LtxA induces NE release from human neutrophils, which subsequently causes the breakdown of periodontal tissues. LtxA-treatment did not induce significant cytotoxicity against human gingival epithelial cells (HGECs) or human gingival fibroblasts (HGFs). However, it did induce significant cytotoxicity against human neutrophils, leading to NE release. Furthermore, NE and the supernatant from LtxA-treated human neutrophils induced detachment and death of HGECs and HGFs, these effects being inhibited by administration of an NE inhibitor, sivelestat. The present results suggest that LtxA mediates human neutrophil lysis and induces the subsequent release of NE, which eventually results in detachment and death of HGECs and HGFs. Thus, LtxA-induced release of NE could cause breakdown of periodontal tissue and thereby exacerbate periodontitis.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_6_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"Microbiology and Immunology. 2019, 63(3-4), 100-110.","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_6_description_53":{"attribute_name":"学位記番号","attribute_value_mlt":[{"subitem_description":"新大院博(歯)第481号","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_6_dissertation_number_52":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲第4873号"}]},"item_6_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1111/1348-0421.12672","subitem_relation_type_select":"DOI"}}]},"item_6_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"【○!C】 2019 The Societies and John Wiley & Sons Australia, Ltd","subitem_rights_language":"en"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Hiyoshi, Takumi","creatorNameLang":"en"},{"creatorName":"日吉, 巧","creatorNameLang":"ja"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"date":[{"dateType":"Available","dateValue":"2022-04-15"}],"displaytype":"detail","filename":"r2ndk481.pdf","filesize":[{"value":"694KB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"本文","objectType":"fulltext","url":"https://niigata-u.repo.nii.ac.jp/record/2000369/files/r2ndk481.pdf"},"version_id":"b903a0dc-c87e-4b66-a83f-585a64e74a7f"},{"date":[{"dateType":"Available","dateValue":"2022-04-15"}],"displaytype":"detail","filename":"r2ndk481_a.pdf","filesize":[{"value":"223KB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"要旨","objectType":"abstract","url":"https://niigata-u.repo.nii.ac.jp/record/2000369/files/r2ndk481_a.pdf"},"version_id":"c1673126-4c13-4666-82fc-8849c3fc3f71"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Aggregatibacter actinomycetemcomitans","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"leukotoxin","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"neutrophil elastase","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"periodontitis","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"Aggregatibacter actinomycetemcomitans induces detachment and death of human gingival epithelial cells and fibroblasts via elastase release following leukotoxin-dependent neutrophil lysis","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Aggregatibacter actinomycetemcomitans induces detachment and death of human gingival epithelial cells and fibroblasts via elastase release following leukotoxin-dependent neutrophil lysis","subitem_title_language":"en"},{"subitem_title":"Aggregatibacter actinomycetemcomitansはロイコトキシンにより好中球を溶解し，エラスターゼを漏出させることで，ヒト歯肉上皮細胞とヒト歯肉線維芽細胞に細胞剥離および細胞死を誘導する","subitem_title_language":"ja"}]},"item_type_id":"6","owner":"1","path":["455","434"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2022-04-15"},"publish_date":"2022-04-15","publish_status":"0","recid":"2000369","relation_version_is_last":true,"title":["Aggregatibacter actinomycetemcomitans induces detachment and death of human gingival epithelial cells and fibroblasts via elastase release following leukotoxin-dependent neutrophil lysis"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2022-12-15T04:13:16.639105+00:00"}