ログイン
言語:

WEKO3

  • トップ
  • ランキング
To
lat lon distance
To

Field does not validate



インデックスリンク

インデックスツリー

メールアドレスを入力してください。

WEKO

One fine body…

WEKO

One fine body…

アイテム

{"_buckets": {"deposit": "f31e9481-dc65-4721-87e1-9f19eee627c2"}, "_deposit": {"id": "2000292", "owners": [1], "pid": {"revision_id": 0, "type": "depid", "value": "2000292"}, "status": "published"}, "_oai": {"id": "oai:niigata-u.repo.nii.ac.jp:02000292", "sets": ["455", "562"]}, "author_link": [], "control_number": "2000292", "item_6_date_granted_51": {"attribute_name": "学位授与年月日", "attribute_value_mlt": [{"subitem_dategranted": "2021-03-23"}]}, "item_6_degree_grantor_49": {"attribute_name": "学位授与機関", "attribute_value_mlt": [{"subitem_degreegrantor": [{"subitem_degreegrantor_language": "ja", "subitem_degreegrantor_name": "新潟大学"}, {"subitem_degreegrantor_language": "en", "subitem_degreegrantor_name": "Niigata University"}], "subitem_degreegrantor_identifier": [{"subitem_degreegrantor_identifier_name": "13101", "subitem_degreegrantor_identifier_scheme": "kakenhi"}]}]}, "item_6_degree_name_48": {"attribute_name": "学位名", "attribute_value_mlt": [{"subitem_degreename": "博士(医学)", "subitem_degreename_language": "ja"}]}, "item_6_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "ATP13A2 is the autosomal recessive causative gene for juvenile-onset Parkinson\u0027s disease (PARK9, Parkinson\u0027s disease 9), also known as Kufor-Rakeb syndrome. The disease is characterized by levodopa-responsive Parkinsonism, supranuclear gaze palsy, spasticity, and dementia. Previously, we have reported that Atp13a2 deficient medaka fish showed dopaminergic neurodegeneration and lysosomal dysfunction, indicating that lysosome-autophagy impairment might be one of the key pathogeneses of Parkinson\u0027s disease. Here, we established Atp13a2 deficient zebrafish using CRISPR/Cas9 gene editing. We found that the number of TH + neurons in the posterior tuberculum and the locus coeruleus significantly reduced (dopaminergic neurons, 64% at 4 months and 37% at 12 months, p \u003c 0.001 and p \u003c 0.05, respectively; norepinephrine neurons, 52% at 4 months and 40% at 12 months, p \u003c 0.001 and p \u003c 0.05, respectively) in Atp13a2 deficient zebrafish, proving the degeneration of dopaminergic neurons. In addition, we found the reduction (60%, p \u003c 0.05) of cathepsin D protein expression in Atp13a2 deficient zebrafish using immunoblot. Transmission electron microscopy analysis using middle diencephalon samples from Atp13a2 deficient zebrafish showed lysosome-like bodies with vesicle accumulation and fingerprint-like structures, suggesting lysosomal dysfunction. Furthermore, a significant reduction (p \u003c 0.001) in protein expression annotated with vesicle fusion with Golgi apparatus in Atp13a2 deficient zebrafish by liquid-chromatography tandem mass spectrometry suggested intracellular trafficking impairment. Therefore, we concluded that Atp13a2 deficient zebrafish exhibited degeneration of dopaminergic neurons, lysosomal dysfunction and the possibility of intracellular trafficking impairment, which would be the key pathogenic mechanism underlying Parkinson\u0027s disease.", "subitem_description_language": "en", "subitem_description_type": "Abstract"}]}, "item_6_description_5": {"attribute_name": "内容記述", "attribute_value_mlt": [{"subitem_description": "IBRO Reports. 2020, 9, 1-8.", "subitem_description_language": "en", "subitem_description_type": "Other"}]}, "item_6_description_53": {"attribute_name": "学位記番号", "attribute_value_mlt": [{"subitem_description": "新大院博(医)第987号", "subitem_description_language": "ja", "subitem_description_type": "Other"}]}, "item_6_dissertation_number_52": {"attribute_name": "学位授与番号", "attribute_value_mlt": [{"subitem_dissertationnumber": "甲第4833号"}]}, "item_6_relation_14": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "https://doi.org/10.1016/j.ibror.2020.05.002", "subitem_relation_type_select": "DOI"}}]}, "item_6_rights_15": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "【○!C】 2020 The Author(s). Published by Elsevier Ltd on behalf of International Brain Research Organization.", "subitem_rights_language": "en"}, {"subitem_rights": "Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International", "subitem_rights_language": "en", "subitem_rights_resource": "https://creativecommons.org/licenses/by-nc-nd/4.0/"}]}, "item_access_right": {"attribute_name": "アクセス権", "attribute_value_mlt": [{"subitem_access_right": "open access", "subitem_access_right_uri": "http://purl.org/coar/access_right/c_abf2"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Nyuzuki, Hiromi", "creatorNameLang": "en"}, {"creatorName": "入月, 浩美", "creatorNameLang": "ja"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"date": [{"dateType": "Available", "dateValue": "2022-04-04"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "r2nmk987.pdf", "filesize": [{"value": "1.95MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": "1.95MB", "url": {"label": "本文", "objectType": "fulltext", "url": "https://niigata-u.repo.nii.ac.jp/record/2000292/files/r2nmk987.pdf"}, "version_id": "20784651-cf25-4ffb-97e7-614b6f0c0043"}, {"date": [{"dateType": "Available", "dateValue": "2022-04-04"}], "displaytype": "detail", "download_preview_message": "", "file_order": 1, "filename": "r2nmk987_a.pdf", "filesize": [{"value": "546KB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": "546KB", "url": {"label": "要旨", "objectType": "abstract", "url": "https://niigata-u.repo.nii.ac.jp/record/2000292/files/r2nmk987_a.pdf"}, "version_id": "9984c474-8202-4c40-9a9b-d2604ee3dfa2"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "Parkinson\u0027s disease", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "ATP13A2", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Zebrafish", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Trafficking impairment", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "doctoral thesis", "resourceuri": "http://purl.org/coar/resource_type/c_db06"}]}, "item_title": "Degeneration of dopaminergic neurons and impaired intracellular trafficking in Atp13a2 deficient zebrafish", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Degeneration of dopaminergic neurons and impaired intracellular trafficking in Atp13a2 deficient zebrafish", "subitem_title_language": "en"}, {"subitem_title": "Atp13a2欠損ゼブラフィッシュにおけるドーパミン神経変性と細胞内輸送障害", "subitem_title_language": "ja"}]}, "item_type_id": "6", "owner": "1", "path": ["455", "562"], "permalink_uri": "http://hdl.handle.net/10191/0002000292", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2022-04-04"}, "publish_date": "2022-04-04", "publish_status": "0", "recid": "2000292", "relation": {}, "relation_version_is_last": true, "title": ["Degeneration of dopaminergic neurons and impaired intracellular trafficking in Atp13a2 deficient zebrafish"], "weko_shared_id": -1}
  1. 0 資料タイプ別
  2. 02 学位論文
  1. 250 大学院医歯学総合研究科(医)
  2. 60 博士学位論文
  3. 10 博士学位論文

Atp13a2欠損ゼブラフィッシュにおけるドーパミン神経変性と細胞内輸送障害

http://hdl.handle.net/10191/0002000292
http://hdl.handle.net/10191/0002000292
f6469641-698d-4aae-9a20-27191043440d
名前 / ファイル ライセンス アクション
r2nmk987.pdf 本文 (1.95MB)
r2nmk987_a.pdf 要旨 (546KB)
Item type 学位論文 / Thesis or Dissertation(1)
公開日 2022-04-04
タイトル
言語 en
タイトル Degeneration of dopaminergic neurons and impaired intracellular trafficking in Atp13a2 deficient zebrafish
タイトル
言語 ja
タイトル Atp13a2欠損ゼブラフィッシュにおけるドーパミン神経変性と細胞内輸送障害
言語
言語 eng
キーワード
言語 en
主題Scheme Other
主題 Parkinson's disease
キーワード
言語 en
主題Scheme Other
主題 ATP13A2
キーワード
言語 en
主題Scheme Other
主題 Zebrafish
キーワード
言語 en
主題Scheme Other
主題 Trafficking impairment
資源タイプ
資源 http://purl.org/coar/resource_type/c_db06
タイプ doctoral thesis
アクセス権
アクセス権 open access
アクセス権URI http://purl.org/coar/access_right/c_abf2
著者 入月, 浩美

× 入月, 浩美

en Nyuzuki, Hiromi

ja 入月, 浩美

Search repository
抄録
内容記述タイプ Abstract
内容記述 ATP13A2 is the autosomal recessive causative gene for juvenile-onset Parkinson's disease (PARK9, Parkinson's disease 9), also known as Kufor-Rakeb syndrome. The disease is characterized by levodopa-responsive Parkinsonism, supranuclear gaze palsy, spasticity, and dementia. Previously, we have reported that Atp13a2 deficient medaka fish showed dopaminergic neurodegeneration and lysosomal dysfunction, indicating that lysosome-autophagy impairment might be one of the key pathogeneses of Parkinson's disease. Here, we established Atp13a2 deficient zebrafish using CRISPR/Cas9 gene editing. We found that the number of TH + neurons in the posterior tuberculum and the locus coeruleus significantly reduced (dopaminergic neurons, 64% at 4 months and 37% at 12 months, p < 0.001 and p < 0.05, respectively; norepinephrine neurons, 52% at 4 months and 40% at 12 months, p < 0.001 and p < 0.05, respectively) in Atp13a2 deficient zebrafish, proving the degeneration of dopaminergic neurons. In addition, we found the reduction (60%, p < 0.05) of cathepsin D protein expression in Atp13a2 deficient zebrafish using immunoblot. Transmission electron microscopy analysis using middle diencephalon samples from Atp13a2 deficient zebrafish showed lysosome-like bodies with vesicle accumulation and fingerprint-like structures, suggesting lysosomal dysfunction. Furthermore, a significant reduction (p < 0.001) in protein expression annotated with vesicle fusion with Golgi apparatus in Atp13a2 deficient zebrafish by liquid-chromatography tandem mass spectrometry suggested intracellular trafficking impairment. Therefore, we concluded that Atp13a2 deficient zebrafish exhibited degeneration of dopaminergic neurons, lysosomal dysfunction and the possibility of intracellular trafficking impairment, which would be the key pathogenic mechanism underlying Parkinson's disease.
言語 en
内容記述
内容記述タイプ Other
内容記述 IBRO Reports. 2020, 9, 1-8.
言語 en
DOI
識別子タイプ DOI
関連識別子 https://doi.org/10.1016/j.ibror.2020.05.002
権利
言語 en
権利情報 【○!C】 2020 The Author(s). Published by Elsevier Ltd on behalf of International Brain Research Organization.
権利
言語 en
権利情報Resource https://creativecommons.org/licenses/by-nc-nd/4.0/
権利情報 Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
学位名
言語 ja
学位名 博士(医学)
学位授与機関
学位授与機関識別子Scheme kakenhi
学位授与機関識別子 13101
言語 ja
学位授与機関名 新潟大学
言語 en
学位授与機関名 Niigata University
学位授与年月日
学位授与年月日 2021-03-23
学位授与番号
学位授与番号 甲第4833号
学位記番号
内容記述タイプ Other
内容記述 新大院博(医)第987号
言語 ja
戻る
0
views
See details
Views

Versions

Ver.1 2022-04-04 06:58:51.926195
Show All versions

Share

Mendeley Twitter Facebook Print Addthis

Cite as

エクスポート

OAI-PMH
  • OAI-PMH JPCOAR
  • OAI-PMH DublinCore
  • OAI-PMH DDI
Other Formats
  • JSON
  • BIBTEX

Confirm


Powered by WEKO3


Powered by WEKO3