{"created":"2022-04-04T06:58:41.916301+00:00","id":2000286,"links":{},"metadata":{"_buckets":{"deposit":"f2d6da5a-32e9-44d0-8a0e-40faa6d1dd69"},"_deposit":{"id":"2000286","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"2000286"},"status":"published"},"_oai":{"id":"oai:niigata-u.repo.nii.ac.jp:02000286","sets":["453:455","471:561:562"]},"author_link":[],"control_number":"2000286","item_6_date_granted_51":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2021-03-23"}]},"item_6_degree_grantor_49":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_language":"ja","subitem_degreegrantor_name":"新潟大学"},{"subitem_degreegrantor_language":"en","subitem_degreegrantor_name":"Niigata University"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"13101","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_6_degree_name_48":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（医学）","subitem_degreename_language":"ja"}]},"item_6_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The generation of mature, functional, thyroid follicular cells from pluripotent stem cells would potentially provide a therapeutic benefit for patients with hypothyroidism, but in vitro differentiation remains difficult. We earlier reported the in vivo generation of lung organs via blastocyst complementation in fibroblast growth factor 10 (Fgf10), compound, heterozygous mutant (Fgf10 Ex1^<mut>/Ex3^<mut>) mice. Fgf10 also plays an essential role in thyroid development and branching morphogenesis but any role thereof in thyroid organogenesis remains unclear. Here, we report that the thyroids of Fgf10 Ex1^<mut>/Ex3^<mut> mice exhibit severe hypoplasia and we generate thyroid tissues from mouse embryonic stem cells (ESCs) in Fgf10 Ex1^<mut>/Ex3^<mut> mice via blastocyst complementation. The tissues were morphologically normal and physiologically functional. The thyroid follicular cells of Fgf10 Ex1^<mut>/Ex3^<mut> chimeric mice were derived largely from GFP-positive mouse ESCs although the recipient cells were mixed. Thyroid generation in vivo via blastocyst complementation will aid functional thyroid regeneration.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_6_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"Front. Endocrinol. 2020, 11, 609697.","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_6_description_53":{"attribute_name":"学位記番号","attribute_value_mlt":[{"subitem_description":"新大院博(医)第981号","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_6_dissertation_number_52":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲第4827号"}]},"item_6_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.3389/fendo.2020.609697","subitem_relation_type_select":"DOI"}}]},"item_6_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"【○!C】 2020 Ran, Zhou, Oda, Yasue, Abe, Ye, Li, Sasaoka, Sakimura, Ajioka and Saijo.","subitem_rights_language":"en"},{"subitem_rights":"Creative Commons Attribution 4.0 International","subitem_rights_language":"en","subitem_rights_resource":"https://creativecommons.org/licenses/by/4.0/"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Ran, Qingsong","creatorNameLang":"en"},{"creatorName":"冉, 慶松","creatorNameLang":"ja"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"date":[{"dateType":"Available","dateValue":"2022-04-04"}],"displaytype":"detail","filename":"r2nmk981.pdf","filesize":[{"value":"1.31MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"本文","objectType":"fulltext","url":"https://niigata-u.repo.nii.ac.jp/record/2000286/files/r2nmk981.pdf"},"version_id":"dcc3f993-aac9-4a1f-9259-6058bfb74f77"},{"date":[{"dateType":"Available","dateValue":"2022-04-04"}],"displaytype":"detail","filename":"r2nmk981_a.pdf","filesize":[{"value":"544KB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"要旨","objectType":"abstract","url":"https://niigata-u.repo.nii.ac.jp/record/2000286/files/r2nmk981_a.pdf"},"version_id":"9544b0d0-b3e0-4e90-8569-9646414d3aba"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"blastocyst complementation","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"embryonic stem cells","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Fgf10","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"pluripotent stem cells","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"thyroid generation","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"Generation of thyroid tissues from embryonic stem cell via blastocyst complementation in vivo","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Generation of thyroid tissues from embryonic stem cell via blastocyst complementation in vivo","subitem_title_language":"en"},{"subitem_title":"胚盤胞補完法とES細胞を用いたマウス生体内における甲状腺再生","subitem_title_language":"ja"}]},"item_type_id":"6","owner":"1","path":["455","562"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2022-04-04"},"publish_date":"2022-04-04","publish_status":"0","recid":"2000286","relation_version_is_last":true,"title":["Generation of thyroid tissues from embryonic stem cell via blastocyst complementation in vivo"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2022-12-15T04:16:28.378684+00:00"}