@article{oai:niigata-u.repo.nii.ac.jp:02000247, author = {Kojima, Kiyohide and 児島, 清秀 and Meguro, Yoko and 目黒, 陽子 and Matsumoto, Tatsuya and 松本, 辰也 and Chino, Shuji and 知野, 秀次}, journal = {新潟大学農学部研究報告, Bulletin of the Faculty of Agriculture Niigata University}, month = {Feb}, note = {To elucidate the site-specific concentration changes of plant hormones associated with various stages of fruit growth inn pears (Pyrus communis cv. 'Le Lectier'), the fruits were harvested 36-177 days after flowering (DAFB), and divided into pericarp, pulp, core, and seed. Indole-3-acetic acid (IAA), trans-zeatin (tZ), isopentenyl adenine (iP), gibberellin_1 (GA_1), gibberellin_4 (GA_4) and abscisic acid (ABA) from each tissue were quantified simultaneously by liquid chromatography mass spectrometry. The IAA concentration in seeds remained higher than that in other tissues during all harvesting periods, and peaked at 123 DAFB. In the pericarp and pulp, the IAA concentrations were almost constant and low and the entire period. In seeds, tZ maintained a higher concentration than other tissues during all harvesting periods, and peaked at 65 and 150 DAFB. The GA_1 concentration was higher than that of GA_4 in all tissues during all periods. The GA_1 concentration in seeds was at 36 DAFB at the highest concentration (406 pmol·g^<-1>FW) and decreased sharply to 65 DAFB. The GA_4 concentration was low and almost constant up to 95 DAFB in seeds but increased after 123 DAFB. The concentrations of core and pulp were low. The fruit core peaked at 123 DAFB and had the highest concentration in the fruit (7610 pmol·g^<-1>FW), with similar changes in the pulp. This study elucidates the concentration changes of the plant hormones at various stages during the growth of 'Le Lectier' fruits., 「ル レクチエ」果実の生長のさまざまな段階に関連する植物ホルモンの部位別の濃度変化を解明するために、開花後36〜177日(DAFBB)に「ル レクチエ」果実を採取し、果皮、果肉、中心部、種子に分けた。各組織からのインドール-3-酢酸(IAA)、トランスゼアチン(tZ)、イソペンテニルアデニン(iP)、ジベレリン_1(GA_1)ジベレリン_4(GA_4)およびアブシジン酸(ABA)を同時に液体クロマトグラフィー質量分析(LC-MS)で定量した。種子中のIAA濃度は、すべての収穫期間中、他の組織よりも高いままであり、123DAFBでピークに達した。果皮と果肉では、IAA濃度はほぼ一定で低い値であった。種子では、tZはすべての収穫期間中、他の組織よりも高い濃度を維持し、65および150DAFBでピークに達した。GA_1濃度は、すべての期間ですべての組織でGA_4の濃度よりも高かった。種子中のGA_1濃度は最高濃度で36DAFBであり、65DAFBまで急激に減少した。GA_4濃度は低く、種子中の95DAFBまではほぼ一定であったが、123DAFB以降は増加した。果芯と果肉の濃度は低かった。果実の芯は123DAFBでピークに達し、果実の濃度が最も高く、果肉にも同様の変化が見られた。}, pages = {11--18}, title = {Site-specific changes in concentrations of endogenous phytohormones in growing 'Le Lectier' fruit}, volume = {74}, year = {2022} }