@article{oai:niigata-u.repo.nii.ac.jp:00019959,
 author = {小野寺, 理},
 issue = {8},
 journal = {新潟医学会雑誌, 新潟医学会雑誌},
 month = {Aug},
 note = {We have devised an improved method for isolating human chromosome specific unique transcribed sequences from a somatic cell hybrid containing only a part human chromosome with use of a primate specific Alu primer. The conditions for the synthesis of heterogenous nuclear RNA complementary DNA were analyzed in detail using HPRT gene as a model system. By increasing the reaction temperature in the first strand synthesis, the specificity of Alu-priming for human-derived sequences was improved. With the improved method, a hncDNA library was construced from a somatic cell hybrid, X3000-11.1, carrying human Xq24-qter as an only human material. After screening the hncDNA library with radiolabeled total human genomic DNA, eleven independent human hncDNA clones were isolated, we generated new human Xq24-qter derived expressed sequence-tagged sites（eSTS）. As the hncDNA clones still contain introns, we screened a human brain cDNA library using 3 Xq28 derived hncDNA clones to isolate cDNA devoid of introns. Two of three hncDNA probes detected positive clones which were devoid of human repetitive sequences. TH4cdNA is located at the centromeric site of Xq28. TH27cDNA is located near the GABRA3 gene. Partial sequence analyses of these clones revealed no homologous sequences reported in GeneBank. The method should be useful to construct physical maps of expressed sequences and isolation of candidate genes for diseases mapped to particular regions of chromosomes.},
 pages = {738--752},
 title = {ヒト染色体特異的Expressed Sequence Tagged Site単離法 : 副腎白質ジストロフィー症の原因遺伝子単離にむけたポジショナルクローニング法によるアプローチ},
 volume = {107},
 year = {1993}
}