{"created":"2021-03-01T06:24:46.871399+00:00","id":19885,"links":{},"metadata":{"_buckets":{"deposit":"1854e055-3d0b-4db1-a721-40ea9e3fe78f"},"_deposit":{"id":"19885","owners":[],"pid":{"revision_id":0,"type":"depid","value":"19885"},"status":"published"},"_oai":{"id":"oai:niigata-u.repo.nii.ac.jp:00019885","sets":["453:456","471:537:538:1158"]},"item_7_alternative_title_1":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Program Freeze and Storage of Hematopoietic Stem Cells from 1979 through 1992 at Niigata University Medical Hospital (Application of Autologous BMT to Cancer Treatment)"}]},"item_7_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1993-09","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"9","bibliographicPageEnd":"798","bibliographicPageStart":"795","bibliographicVolumeNumber":"107","bibliographic_titles":[{"bibliographic_title":"新潟医学会雑誌"},{"bibliographic_title":"新潟医学会雑誌","bibliographic_titleLang":"en"}]}]},"item_7_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Our experience of a slow rate freezing of hematopoietic stem cells during 13 years is described. Harvested bone marrows were concentrated in nucleated cell rich fraction using automatic blood separators. Total 182 stem cells including 79 peripheral leukocytes in chronic myelogenous leukemia （CML）, 93 bone marrow cells in leukemias/lymphomas, gynecological and urological neoplasmas and brain tumors and 10 peripheral blood stem cells in pediatric diseases were cryopreserved in the presence of 10% dimethyl sufoxide. Freezing of circulating leukocytes in CML has been discontinued since 1981 because of eventual blastic crisis. All cells were frozen on the same program in which they were cooled at 1℃ per minute rate from 20℃ to －40℃ and then 3℃ per minute rate to －80℃. By picking up a critical freezing point of the sample and cooling the chamber, our program freezer （Ebara, RF-22R2）has always produced straight lines in lowering sample temperature. Frozen cells were kept in a liquid nitrogen stocker until their infusion. Controling sample and chamber temperature profiles is necessary for a good course after transplantation.","subitem_description_type":"Abstract"}]},"item_7_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"123244","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Shinada, Shoji"}]}]},"item_7_publisher_7":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"新潟医学会"}]},"item_7_select_19":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"publisher"}]},"item_7_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00182415","subitem_source_identifier_type":"NCID"}]},"item_7_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"00290440","subitem_source_identifier_type":"ISSN"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"品田, 章二"}],"nameIdentifiers":[{"nameIdentifier":"123243","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-08-16"}],"displaytype":"detail","filename":"107(9)_795-798.pdf","filesize":[{"value":"656.8 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"107(9)_795-798.pdf","url":"https://niigata-u.repo.nii.ac.jp/record/19885/files/107(9)_795-798.pdf"},"version_id":"eb3c39b9-ba01-4d34-b4f6-523226f94cce"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"slow rate freezing","subitem_subject_scheme":"Other"},{"subitem_subject":"hematopoietic stem cell","subitem_subject_scheme":"Other"},{"subitem_subject":"dimethyl sulfoxide","subitem_subject_scheme":"Other"},{"subitem_subject":"liquid nitrogen stocker","subitem_subject_scheme":"Other"},{"subitem_subject":"straight line in lowering","subitem_subject_scheme":"Other"},{"subitem_subject":"緩徐凍結","subitem_subject_scheme":"Other"},{"subitem_subject":"造血幹細胞","subitem_subject_scheme":"Other"},{"subitem_subject":"ジメチルスルフォキサイド","subitem_subject_scheme":"Other"},{"subitem_subject":"液体窒素槽","subitem_subject_scheme":"Other"},{"subitem_subject":"直線状の温度低下","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"2）造血幹細胞の凍結・保存(シンポジウム 自家骨髄移植の応用, 第482回新潟医学会)","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"2）造血幹細胞の凍結・保存(シンポジウム 自家骨髄移植の応用, 第482回新潟医学会)"},{"subitem_title":"2）造血幹細胞の凍結・保存(シンポジウム 自家骨髄移植の応用, 第482回新潟医学会)","subitem_title_language":"en"}]},"item_type_id":"7","owner":"1","path":["456","1158"],"pubdate":{"attribute_name":"公開日","attribute_value":"2016-02-05"},"publish_date":"2016-02-05","publish_status":"0","recid":"19885","relation_version_is_last":true,"title":["2）造血幹細胞の凍結・保存(シンポジウム 自家骨髄移植の応用, 第482回新潟医学会)"],"weko_creator_id":"1","weko_shared_id":null},"updated":"2022-12-15T03:50:46.302492+00:00"}