WEKO3
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2)造血幹細胞の凍結・保存(シンポジウム 自家骨髄移植の応用, 第482回新潟医学会)
http://hdl.handle.net/10191/37676
http://hdl.handle.net/10191/37676dbc2c125-b575-4ef0-8d09-29ce0dce8b52
名前 / ファイル | ライセンス | アクション |
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2016-02-05 | |||||
タイトル | ||||||
タイトル | 2)造血幹細胞の凍結・保存(シンポジウム 自家骨髄移植の応用, 第482回新潟医学会) | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | 2)造血幹細胞の凍結・保存(シンポジウム 自家骨髄移植の応用, 第482回新潟医学会) | |||||
言語 | ||||||
言語 | jpn | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | slow rate freezing | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | hematopoietic stem cell | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | dimethyl sulfoxide | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | liquid nitrogen stocker | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | straight line in lowering | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 緩徐凍結 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 造血幹細胞 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | ジメチルスルフォキサイド | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 液体窒素槽 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 直線状の温度低下 | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | departmental bulletin paper | |||||
その他のタイトル | ||||||
その他のタイトル | Program Freeze and Storage of Hematopoietic Stem Cells from 1979 through 1992 at Niigata University Medical Hospital (Application of Autologous BMT to Cancer Treatment) | |||||
著者 |
品田, 章二
× 品田, 章二 |
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著者別名 | ||||||
識別子 | 123244 | |||||
識別子Scheme | WEKO | |||||
姓名 | Shinada, Shoji | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Our experience of a slow rate freezing of hematopoietic stem cells during 13 years is described. Harvested bone marrows were concentrated in nucleated cell rich fraction using automatic blood separators. Total 182 stem cells including 79 peripheral leukocytes in chronic myelogenous leukemia (CML), 93 bone marrow cells in leukemias/lymphomas, gynecological and urological neoplasmas and brain tumors and 10 peripheral blood stem cells in pediatric diseases were cryopreserved in the presence of 10% dimethyl sufoxide. Freezing of circulating leukocytes in CML has been discontinued since 1981 because of eventual blastic crisis. All cells were frozen on the same program in which they were cooled at 1℃ per minute rate from 20℃ to -40℃ and then 3℃ per minute rate to -80℃. By picking up a critical freezing point of the sample and cooling the chamber, our program freezer (Ebara, RF-22R2)has always produced straight lines in lowering sample temperature. Frozen cells were kept in a liquid nitrogen stocker until their infusion. Controling sample and chamber temperature profiles is necessary for a good course after transplantation. | |||||
書誌情報 |
新潟医学会雑誌 en : 新潟医学会雑誌 巻 107, 号 9, p. 795-798, 発行日 1993-09 |
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出版者 | ||||||
出版者 | 新潟医学会 | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 00290440 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AN00182415 | |||||
著者版フラグ | ||||||
値 | publisher |