@article{oai:niigata-u.repo.nii.ac.jp:00019884,
 author = {森山, 美昭 and 岸, 賢治 and 後藤, 隆夫 and 橋本, 誠雄},
 issue = {9},
 journal = {新潟医学会雑誌, 新潟医学会雑誌},
 month = {Sep},
 note = {In order to apply a simple purging method by heat to autologous bone marrow transplantation （ABMT）, we have revaluated the ability to purge clonogenic leukemic cells from the simulated marrow mixture of normal marrow cells and leukemic cell lines （HL60, Molt-3 and HEL）in vitro by heat, using two different clonogenic assays for normal granulocyte-macrophage progenitors （CFU-GM） and leukemic cell lines. The cytotoxic effect of cryopreservation on heat-treated normal marrow cells in vitro was also examined. It appeared that in vitro hyperthermia （42℃ for 120 min） is able to selectively remove clonogenic leukemic cells from simulated tumor cell-normal marrow mixtures even when leukemic cell concentrations are increased up to 3×10^6 cells/ml in vitro, and results in a 4-to-6 log destruction of clonogenic leukemic cells/ml according to a limiting dilution assay, while leaving half of normal CFU-GM surviving. The hyperthemic purging of clonogenic leukemic cells was not affected in the presence of normal marrow cells in vitro. This high level of clonogenic leukemic cell depletion by heat correlated with that of immunologic and pharmacologic studies. In addition, treatment of normal or remission marrow cells with hyperthermia did not significantly change the growth of CFU-GM after cryopreservation, and the percentage recovery of CFU-GM ranged from 34 to 79%. These results suggest that in vitro hyperthermia could be applied effectively and safely for the elimination of residual clonogenic leukemic cells in autologous marrow grafts before ABMT.},
 pages = {790--794},
 title = {1）Ex vivo marrow purging : 熱処理による腫瘍細胞の除去とそのシミュレーション実験(シンポジウム 自家骨髄移植の応用, 第482回新潟医学会)},
 volume = {107},
 year = {1993}
}