@article{oai:niigata-u.repo.nii.ac.jp:00019817,
 author = {辻, 省次},
 issue = {11},
 journal = {新潟医学会雑誌, 新潟医学会雑誌},
 month = {Nov},
 note = {Recent advances in molecular genetics have made it possible to identify genes for various hereditary neurodegenerative disorders. The approach, which is now called positional cloning, consists of two steps. The first step is to determine the chromosomal localizations of disease genes by linkage analyses. The second step is to determine the causative genes themselves. As many microsatellite markers have been described, the linkage analysis can now be performed far more effectively. Development of new technologies including pulsed-field gel electrophoresis and cloning of several hundred kilobases of genomic DNA using yeast artificial chromosomes have also brought new effective strategies for the positional cloning. Our laboratory has been involved in identification of genes for neurologic diseases including adrenoleukodystrophy and various forms of spinocerebellar degenerations. Application of microsatellite polymorphisms for the linkage analysis of early-onset ataxia associated with hypoalbuminemia (EOAHA) was described. As a new technology for positional cloning, an improved hncDNA (heterogenous nuclear RNA-complementary DNA) cloning method was described. Our approaches toward the identification of the causative genes for these diseases and better understanding of the molecular mechanisms of neurodegenerative diseases are discussed.},
 pages = {972--980},
 title = {1) 神経疾患の分子メカニズム(新潟大学脳研究所創立25周年記念講演会)},
 volume = {107},
 year = {1993}
}