@article{oai:niigata-u.repo.nii.ac.jp:00019481,
 author = {吉田, 俊明 and 早川, 晃史 and 伊藤, 信市 and 上村, 朝輝 and 朝倉, 均 and 石原, 清},
 issue = {3},
 journal = {新潟医学会雑誌, 新潟医学会雑誌},
 month = {Mar},
 note = {Antimitochondrial antibodies, serological hallmarks of primary biliary cirrhosis, recently were found to be directed against the E2 subunits of mitochondrial dehydrogenase complexes. The first objective of this study was to extend these findings and to determine whether purified immunoglobulin from the sera of patients with primary biliary cirrhosis inhibit activity of these dehydrogenase complexes in vitro. The second objective was to confirm whether the detection of antibodies against mitochondrial dehydrogenase complexes contributes the diagnosis of primary biliary cirrhosis and prediction of prognosis of the disease. Purified immunoglobulin from 13 patients with various titer of mitochondrial antibodies showed concentration-dependent inhibition of activity of the dehydrogenase complexes, and close correlation was observed between inhibitory activity against pyruvate dehydrogenase complex and the reciprocal titer of immunoglobulin against this complex. Of 100 patients with primary biliary cirrhosis, 79, 77 and 24 patients showed positive test for antibody against pyruvate dehydrogenase, branched-chain ketoacid dehydrogenase, and alpha-ketoglutarate dehydrogenase complex, respectively by enzyme linked immunosorbent assay. Only 7 patients with primary biliary cirrhosis showed none of positive test for antibodies against these enzyme complexes. Patients with positive for antibody against pyruvate dehydrogenase complex showed higher level of serum cholesterol and bilirubin than those with negative. Furthermore, all of the patients with positive test for antibodies against pyruvate dehydrogenase complex showed early stage of liver histology. We conclude that the detection of antibody against pyruvate dehydrogenase complex, besides serving as diagnostic markers for primary biliary cirrhosis, may contribute to predict the prognosis of patient with this disease.},
 pages = {185--189},
 title = {3) 原発性胆汁性肝硬変(シンポジウム 自己免疫疾患の新しい病態, 第487回新潟医学会)},
 volume = {108},
 year = {1994}
}