{"created":"2021-03-01T06:24:00.580059+00:00","id":19179,"links":{},"metadata":{"_buckets":{"deposit":"365c1ccb-8f5f-43f7-87d7-41ca8f172ca0"},"_deposit":{"id":"19179","owners":[],"pid":{"revision_id":0,"type":"depid","value":"19179"},"status":"published"},"_oai":{"id":"oai:niigata-u.repo.nii.ac.jp:00019179","sets":["453:456","471:537:538:1147"]},"item_7_alternative_title_1":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"The Study in the Oncogeneity of the Chimeric P210BCR-ABL Gene by Using Human Hematopoietic Cell Line and Transgenic Mouse"}]},"item_7_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1994-08","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"8","bibliographicPageEnd":"592","bibliographicPageStart":"579","bibliographicVolumeNumber":"108","bibliographic_titles":[{"bibliographic_title":"新潟医学会雑誌"},{"bibliographic_title":"新潟医学会雑誌","bibliographic_titleLang":"en"}]}]},"item_7_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"In order to investigate how the chimeric BCR-ABL gene is involved in the pathogenesis of human leukemia, we introduced a P210BCR-ABL and a BCR-v-abl gene into HSM-911, a human IL-3 or GM-CSF dependent myeloid cell line, and a P210BCR-ABL gene into the mouse germ line. In experiments in vitro, clones of HSM-911 into which a BCR-v-abl gene was introduced revealed abrogation of IL-3 dependence, but those with a P210BCR-ABL gene did not proliferate independently of exogenous growth factors. Three of 7 P210BCR-ABL clones, however, acquired the ability to differentiate into mature cells. This results may indicate that the expression of P210BCR-ABL gene is associated with the maturation of cells established as blastic cell line from a leukemic patient, which is in accordance with the nature of terminal differentiation of granulocytes in chronic phase of CML. Next, we have successfully generated mice transgenic for a P210BCR-ABL gene under the control of the MPSV pormoter elements. Expression of the P210BCR-ABL mRNA was detected in almost every organ of mice analysed by RT-PCR. One of 12 founder mice died 90 days after birth, showing midgrade leukothrombocytosis with a prominent increase in the granulocytic lineage and splenomegaly. Pathological analysis showed disappearance of normal splenic structure and infiltration of cells in each maturation stages of three hematopoietic components, that is the findings characteristic of CML. Although this suggests that the P210BCR-ABL gene plays a crutial role in early stages of CML, the low incidence of the disease in our experiment may indicate that the level of P210BCR-ABL gene expression is related to the first step and further developments such as acceleration of CML require other factors, e.g. additional genetic events.","subitem_description_type":"Abstract"}]},"item_7_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"119443","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Nikkuni, Koji"}]}]},"item_7_publisher_7":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"新潟医学会"}]},"item_7_select_19":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"publisher"}]},"item_7_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00182415","subitem_source_identifier_type":"NCID"}]},"item_7_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"00290440","subitem_source_identifier_type":"ISSN"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"新國, 公司"}],"nameIdentifiers":[{"nameIdentifier":"119442","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-08-08"}],"displaytype":"detail","filename":"108(8)_579-592.pdf","filesize":[{"value":"5.0 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"108(8)_579-592.pdf","url":"https://niigata-u.repo.nii.ac.jp/record/19179/files/108(8)_579-592.pdf"},"version_id":"3ceb0a77-768c-47e1-a66c-aca3e778681c"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"CML","subitem_subject_scheme":"Other"},{"subitem_subject":"Ph^1 chromosome","subitem_subject_scheme":"Other"},{"subitem_subject":"P210BCR-ABL gene","subitem_subject_scheme":"Other"},{"subitem_subject":"transgenic mice","subitem_subject_scheme":"Other"},{"subitem_subject":"慢性骨髄性白血病","subitem_subject_scheme":"Other"},{"subitem_subject":"Ph^1 染色体","subitem_subject_scheme":"Other"},{"subitem_subject":"P210BCR-ABL 遺伝子","subitem_subject_scheme":"Other"},{"subitem_subject":"トランスジェニックマウス","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"ヒト血液細胞株及びトランスジェニックマウスを用いたBCR-ABL遺伝子の造腫瘍性に関する検討","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"ヒト血液細胞株及びトランスジェニックマウスを用いたBCR-ABL遺伝子の造腫瘍性に関する検討"},{"subitem_title":"ヒト血液細胞株及びトランスジェニックマウスを用いたBCR-ABL遺伝子の造腫瘍性に関する検討","subitem_title_language":"en"}]},"item_type_id":"7","owner":"1","path":["456","1147"],"pubdate":{"attribute_name":"公開日","attribute_value":"2016-02-18"},"publish_date":"2016-02-18","publish_status":"0","recid":"19179","relation_version_is_last":true,"title":["ヒト血液細胞株及びトランスジェニックマウスを用いたBCR-ABL遺伝子の造腫瘍性に関する検討"],"weko_creator_id":"1","weko_shared_id":null},"updated":"2022-12-15T03:50:05.263241+00:00"}