@article{oai:niigata-u.repo.nii.ac.jp:00018891, author = {桑野, 良三}, issue = {11}, journal = {新潟医学会雑誌, 新潟医学会雑誌}, month = {Nov}, note = {Spot 35 protein was first discovered from the cerebellum as a specific protein. Immunohistochemistry demonstrated wide distribution of this protein in the Purkinje cells, intestine, sensory organs and kidney. cDNA cloning and nucleotide sequence showed that Spot 35 protein is identical with calbindin D 28k, a vitamin D dependent calcium binding protein. Two vitamin D response elements (pDRE and dDRE) were found in the 5' upstream region of the Spot 35/Calbindin D 28k gene. Promoter activities were determined by using luciferase as a reporter gene in MDBK cells which produced endogenously Spot 35/Calbindin D 28k. Transcription of the chimeric gene containing the pDRE was enhanced by 1, 25 (OH)_2D_3 stimulation in an orientation-dependent fashion.}, pages = {798--803}, title = {Spot 35/calbindin D 28k遺伝子の解析}, volume = {108}, year = {1994} }