@article{oai:niigata-u.repo.nii.ac.jp:00017214,
 author = {月俣, ジャイメ耕一},
 issue = {8},
 journal = {新潟医学会雑誌, 新潟医学会雑誌},
 month = {Aug},
 note = {One of the most severe forms of myocarditis is giant cell myocarditis, because it has a poor prognosis with occurrence of fatal arrhythmias and it may develop into dilated cardiomyopathy (DCM) through immuno-mediated myocadial damage. Experimental autoimmune myocarditis (EAM) in rats is a model of giant cell myocarditis which resembles that of giant cell myocarditis in humans. In order to investigate the possible role of TGF-β1 in the accumulation of extracellular matrix (ECM) proteins in rats with EAM, we examined the expression of TGF-β1, types I and II TGF-β receptors and ECM proteins (fibronectin, α1 type I collagen, α1 type III collagen and α2 type IV collagen) in the heart during 60 days of the EAM course by ribonuclease protection assay and immunostaining. Furthermore we performed ribonuclease protection assay of collagenase 72, collagenase 92 and TIMP-1. TGF-β1 mRNA expression markedly increased on Days 16 and 21 in parallel to an increase in mRNA expression of type I and II TGF-β receptors, suggesting the interdependence of type I and II TGF-β receptors for signal transduction. Immunofluorescence microscopy showed the presence of TGF-β1 in some macrophages but not in all infiltrating in the hearts. Type I TGF-β receptor was immunostained in the interstitium of the heart. An increase in mRNA expression of fibronectin, type I collagen, type III collagen and type IV collagen was also observed from Day 16 to Day 42. The expression of mRNA for type I collagen and type IV collagen decreased on Day 60 whereas fibronectin and type III collagen mRNA were still elevated on Day 60 in the EAM hearts. The changes in the mRNA expression were arbitrarily comparable to the expansion of ECM deposition detected by immunofluorescence. The expression of mRNA for collagenase 72 and collagenase 92 which degrade type IV collagen, was increased in the control group, reached their peaks on Day 30 and decreased thereafter. TIMP-1 mRNA expression markedly increased on Day 16, decreased slightly on Day 21, remained unchanged on Day 30, and decreased sharply on Day 42 and 60. These results may indicate that TGF-β1 along with TGF-β receptors play a crucial role in the expression and deposition of ECM components in EAM hearts.},
 pages = {509--522},
 title = {ラット実験的自己免疫性心筋炎の拡張型心筋症に進展する機序の検討 : TGF-β1, TGF-β受容体, 細胞外基質蛋白, Collagenase 72, Collagenase 92およびTIMP-1の発現について},
 volume = {111},
 year = {1997}
}