{"created":"2021-03-01T06:21:35.221544+00:00","id":16952,"links":{},"metadata":{"_buckets":{"deposit":"8e363174-85c1-4699-a9ba-9f8b7689eb24"},"_deposit":{"id":"16952","owners":[],"pid":{"revision_id":0,"type":"depid","value":"16952"},"status":"published"},"_oai":{"id":"oai:niigata-u.repo.nii.ac.jp:00016952","sets":["453:456","471:537:538:1114"]},"item_7_alternative_title_1":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Effects of Temperature and pH on Changes in Intracelluar Ca Ion Concentration of Rat Hippocampal Slices during Oxygen-Glucose Deprivation"}]},"item_7_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1997-11","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"11","bibliographicPageEnd":"712","bibliographicPageStart":"705","bibliographicVolumeNumber":"111","bibliographic_titles":[{"bibliographic_title":"新潟医学会雑誌"},{"bibliographic_title":"新潟医学会雑誌","bibliographic_titleLang":"en"}]}]},"item_7_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Low temperature is commonly known to protect neurons against ischemia, and neuroprotective effect of mild acidosis has also been suggested in our recent reports. In this study, the effects of temperature and pH on ischemic cell damage were studied in terms of intracellular Ca^<2+>, as an indicator of ischemic cell damage, using rat hippocampal slice preparations. Intracellular Ca^<2+> concentration was measured by fluorometry using fura-2. Slices were exposed to ischemia by perfusion with glucose-free artificial CSF (ACSF) bubbled with 95% N_2-5% CO_2. Intracelluar Ca^<2+> showed a characteristic rapid increase at 334±83 sec (n=24) following the start of ischemia at normal temperature (37℃). The latency of the characteristic rapid increase in intracellular Ca^<2+> was prolonged to 535±49 sec (n=9) at low temperature (33℃), while it was shortened to 189±39 sec (n=12) at high temperature (42℃). The latency of the rapid intracellular Ca2+ increase was shortened at each temperature in alkalized ACSF (pH 7.8). The characteristic rapid increase in intracellar Ca^<2+> was not observed at each temperature in acidified ACSF (pH 6.8). The results indicate that both temperature and pH affect the time to the rapid increase in intracellular Ca^<2+> in ischemic tissue, and suggest the neuroprotective effect of hypothermia as well as mild acidosis and also hypothermia plus mild acidosis.","subitem_description_type":"Abstract"}]},"item_7_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"107357","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Ebine, Yoshiko"}]}]},"item_7_publisher_7":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"新潟医学会"}]},"item_7_select_19":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"publisher"}]},"item_7_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00182415","subitem_source_identifier_type":"NCID"}]},"item_7_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"00290440","subitem_source_identifier_type":"ISSN"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"海老根, 美子"}],"nameIdentifiers":[{"nameIdentifier":"107356","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-08-08"}],"displaytype":"detail","filename":"111(11)_705-712.pdf","filesize":[{"value":"1.8 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"111(11)_705-712.pdf","url":"https://niigata-u.repo.nii.ac.jp/record/16952/files/111(11)_705-712.pdf"},"version_id":"dd20dd4c-ee03-4e03-9506-0f396aac5e54"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"pH","subitem_subject_scheme":"Other"},{"subitem_subject":"temperature","subitem_subject_scheme":"Other"},{"subitem_subject":"intracellular Ca ion concentration","subitem_subject_scheme":"Other"},{"subitem_subject":"ischemia","subitem_subject_scheme":"Other"},{"subitem_subject":"oxygen-glucose deprivation","subitem_subject_scheme":"Other"},{"subitem_subject":"温度","subitem_subject_scheme":"Other"},{"subitem_subject":"細胞内 Ca イオン濃度","subitem_subject_scheme":"Other"},{"subitem_subject":"虚血","subitem_subject_scheme":"Other"},{"subitem_subject":"低酸素・無グルコース","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"低酸素・無グルコース負荷中のラット海馬切片細胞内Caイオン濃度上昇に対する温度とpHの影響","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"低酸素・無グルコース負荷中のラット海馬切片細胞内Caイオン濃度上昇に対する温度とpHの影響"},{"subitem_title":"低酸素・無グルコース負荷中のラット海馬切片細胞内Caイオン濃度上昇に対する温度とpHの影響","subitem_title_language":"en"}]},"item_type_id":"7","owner":"1","path":["456","1114"],"pubdate":{"attribute_name":"公開日","attribute_value":"2017-01-13"},"publish_date":"2017-01-13","publish_status":"0","recid":"16952","relation_version_is_last":true,"title":["低酸素・無グルコース負荷中のラット海馬切片細胞内Caイオン濃度上昇に対する温度とpHの影響"],"weko_creator_id":"1","weko_shared_id":null},"updated":"2022-12-15T03:48:24.988024+00:00"}