@article{oai:niigata-u.repo.nii.ac.jp:00016952,
 author = {海老根, 美子},
 issue = {11},
 journal = {新潟医学会雑誌, 新潟医学会雑誌},
 month = {Nov},
 note = {Low temperature is commonly known to protect neurons against ischemia, and neuroprotective effect of mild acidosis has also been suggested in our recent reports. In this study, the effects of temperature and pH on ischemic cell damage were studied in terms of intracellular Ca^<2+>, as an indicator of ischemic cell damage, using rat hippocampal slice preparations. Intracellular Ca^<2+> concentration was measured by fluorometry using fura-2. Slices were exposed to ischemia by perfusion with glucose-free artificial CSF (ACSF) bubbled with 95% N_2-5% CO_2. Intracelluar Ca^<2+> showed a characteristic rapid increase at 334±83 sec (n=24) following the start of ischemia at normal temperature (37℃). The latency of the characteristic rapid increase in intracellular Ca^<2+> was prolonged to 535±49 sec (n=9) at low temperature (33℃), while it was shortened to 189±39 sec (n=12) at high temperature (42℃). The latency of the rapid intracellular Ca2+ increase was shortened at each temperature in alkalized ACSF (pH 7.8). The characteristic rapid increase in intracellar Ca^<2+> was not observed at each temperature in acidified ACSF (pH 6.8). The results indicate that both temperature and pH affect the time to the rapid increase in intracellular Ca^<2+> in ischemic tissue, and suggest the neuroprotective effect of hypothermia as well as mild acidosis and also hypothermia plus mild acidosis.},
 pages = {705--712},
 title = {低酸素・無グルコース負荷中のラット海馬切片細胞内Caイオン濃度上昇に対する温度とpHの影響},
 volume = {111},
 year = {1997}
}