{"created":"2021-03-01T06:21:35.086178+00:00","id":16950,"links":{},"metadata":{"_buckets":{"deposit":"680c582b-2a11-40ea-b310-2cc4f74565c4"},"_deposit":{"id":"16950","owners":[],"pid":{"revision_id":0,"type":"depid","value":"16950"},"status":"published"},"_oai":{"id":"oai:niigata-u.repo.nii.ac.jp:00016950","sets":["453:456","471:537:538:1114"]},"item_7_alternative_title_1":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Dominant Appearance of Normal Hematopoietic Progenitors in the Adherent Layer of Long-Term Marrow Culture in Chronic Myelogenous Leukemia : An Effect of Interferon-α"}]},"item_7_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1997-11","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"11","bibliographicPageEnd":"690","bibliographicPageStart":"683","bibliographicVolumeNumber":"111","bibliographic_titles":[{"bibliographic_title":"新潟医学会雑誌"},{"bibliographic_title":"新潟医学会雑誌","bibliographic_titleLang":"en"}]}]},"item_7_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"As an alternative treatment with allogeneic bone marrow transplantation, a possibility of autologous stem cell transplantation for chronic myelogenous leukemia (CML) was studied. The most critical problem in this study is how to purify normal stem cells from autologous bone marrow or peripheral blood cells. Although normal stem cells are present in these patients in chronic phase, absolute amount of these cells are very small and difficult to detect by regular chromosome analysis. Concerning to a previous report, appearance of Philadelphia chromosome (Ph) negative clones was observed after long-term marrow culture (LTMC) of CML patients. Our previous studies had shown that addition of interferon-α (IFNα) in LTMC preferentially decrease Ph cells. In the present study, we investigated the frequencies of leukemic clones in suspending-and adherent-fraction of LTMC by reverse transcriptional-polymerase chain reaction (RT-PCR) for bcr-abl, CML specific and chromosome analysis of a single progenitor derived colony. All colonies derived from suspending cells presented either bcr-abl and Ph chromosome in both cultures with and without IFNα. In all 11 colonies derived from adherent-fraction of LTMC without IFNα, bcr-abl was detectable, whereas 2/2 CFU-mix and 3/11 CFU-GM were negative for bcr-abl from the culture with IFNα. Results from chromosome analysis of single colony was similar to that by RT-PCR. These results indicate the possibility of application of LTMC with IFNα to eliminate leukemic clones from CML marrow cells, however, there remains a problem that stem cell number decreases during LTMC with IFNα. Our investigation of ex vivo expansion for human hematopoietic stem cells is still experimental, and more progression is necessary for clinical application.","subitem_description_type":"Abstract"}]},"item_7_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"107348","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Suzuki, Noriatsu"}]},{"nameIdentifiers":[{"nameIdentifier":"107349","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Kishi, Kenji"}]},{"nameIdentifiers":[{"nameIdentifier":"107350","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Narita, Miwako"}]},{"nameIdentifiers":[{"nameIdentifier":"107351","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Furukawa, Tatsuo"}]},{"nameIdentifiers":[{"nameIdentifier":"107352","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Takahashi, Masuhiro"}]},{"nameIdentifiers":[{"nameIdentifier":"107353","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Aizawa, Yoshifusa"}]}]},"item_7_publisher_7":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"新潟医学会"}]},"item_7_select_19":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"publisher"}]},"item_7_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00182415","subitem_source_identifier_type":"NCID"}]},"item_7_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"00290440","subitem_source_identifier_type":"ISSN"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"鈴木, 訓充"}],"nameIdentifiers":[{"nameIdentifier":"107342","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"岸, 賢治"}],"nameIdentifiers":[{"nameIdentifier":"107343","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"成田, 美和子"}],"nameIdentifiers":[{"nameIdentifier":"107344","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"古川, 達雄"}],"nameIdentifiers":[{"nameIdentifier":"107345","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"高橋, 益広"}],"nameIdentifiers":[{"nameIdentifier":"107346","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"相澤, 義房"}],"nameIdentifiers":[{"nameIdentifier":"107347","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-08-08"}],"displaytype":"detail","filename":"111(11)_683-690.pdf","filesize":[{"value":"1.4 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"111(11)_683-690.pdf","url":"https://niigata-u.repo.nii.ac.jp/record/16950/files/111(11)_683-690.pdf"},"version_id":"8b5dfbe5-9fbc-49ca-afac-1a27e7f09578"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"LTMC","subitem_subject_scheme":"Other"},{"subitem_subject":"IFNα","subitem_subject_scheme":"Other"},{"subitem_subject":"CML","subitem_subject_scheme":"Other"},{"subitem_subject":"bcr-abl","subitem_subject_scheme":"Other"},{"subitem_subject":"Philadelphia chromosome","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"慢性骨髄性白血病における骨髄長期培養では正常前駆細胞は付着細胞層に多く存在する : インターフェロンの効果","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"慢性骨髄性白血病における骨髄長期培養では正常前駆細胞は付着細胞層に多く存在する : インターフェロンの効果"},{"subitem_title":"慢性骨髄性白血病における骨髄長期培養では正常前駆細胞は付着細胞層に多く存在する : インターフェロンの効果","subitem_title_language":"en"}]},"item_type_id":"7","owner":"1","path":["456","1114"],"pubdate":{"attribute_name":"公開日","attribute_value":"2017-01-13"},"publish_date":"2017-01-13","publish_status":"0","recid":"16950","relation_version_is_last":true,"title":["慢性骨髄性白血病における骨髄長期培養では正常前駆細胞は付着細胞層に多く存在する : インターフェロンの効果"],"weko_creator_id":"1","weko_shared_id":null},"updated":"2022-12-15T03:48:24.748247+00:00"}