{"created":"2021-03-01T06:21:35.019089+00:00","id":16949,"links":{},"metadata":{"_buckets":{"deposit":"e1fc59dd-4bf9-4829-bc60-56766818bc69"},"_deposit":{"id":"16949","owners":[],"pid":{"revision_id":0,"type":"depid","value":"16949"},"status":"published"},"_oai":{"id":"oai:niigata-u.repo.nii.ac.jp:00016949","sets":["453:456","471:537:538:1114"]},"item_7_alternative_title_1":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Pathogenetic Analysis of a Platelet Disorder Characterized by the Absence of Thromboxane A_2-Induced Platelet Aggregation"}]},"item_7_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1997-11","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"11","bibliographicPageEnd":"682","bibliographicPageStart":"676","bibliographicVolumeNumber":"111","bibliographic_titles":[{"bibliographic_title":"新潟医学会雑誌"},{"bibliographic_title":"新潟医学会雑誌","bibliographic_titleLang":"en"}]}]},"item_7_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"A patient with a mild bleeding disorder whose platelets responded defectively to thromboxane A_2 (TXA_2) was identified, and the mechanism of this dysfunction was analyzed. The platelets were defective in aggregation and release reaction in response to synthetic TXA_2 mimetic (STA_2). When the platelet TXA_2 receptor was examined with both a [^<125>I]-labeled derivative of a TXA_2 receptor antagonist ([^<125>I]-PTAOH) and [＾3H]-labeled TXA_2 agonist ([^3H]U-46619), the equilibrium dissociation rate constants(Kd)and the maximal concentrations of binding sites (Bmax) of the platelets to both ligands were within normal ranges, suggesting that the binding capacity of their TXA_2 receptor was normal. STA_2 could not induce IP3 formation and intracellular Ca^<2+> mobilization, whereas these responses to thrombin were within normal ranges. GTP ase activity was also decreased when the patient's platelet membrane was challenged with STA_2. Furthermore, we identified a single amino acid substitution (Arg^<60>→Leu) in the first cytoplasmic loop of the patient's platelet TXA_2 receptor. The mutant receptor expressed in Chinese hamster ovary cells showed decreased TXA_2-induced second messenger formation despite its normal ligand binding affinities. These results suggest that the Arg^<60> to Leu mutation is responsible for the disorder and that this region could also comprise an important functional element for interaction with G protein linked to PLC.","subitem_description_type":"Abstract"}]},"item_7_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"107341","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Fuse, Ichiro"}]}]},"item_7_publisher_7":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"新潟医学会"}]},"item_7_select_19":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"publisher"}]},"item_7_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00182415","subitem_source_identifier_type":"NCID"}]},"item_7_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"00290440","subitem_source_identifier_type":"ISSN"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"布施, 一郎"}],"nameIdentifiers":[{"nameIdentifier":"107340","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-08-08"}],"displaytype":"detail","filename":"111(11)_676-682.pdf","filesize":[{"value":"1.1 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"111(11)_676-682.pdf","url":"https://niigata-u.repo.nii.ac.jp/record/16949/files/111(11)_676-682.pdf"},"version_id":"6aeb0001-cf62-452d-ac04-824ca0a14128"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"platelets","subitem_subject_scheme":"Other"},{"subitem_subject":"thromboxane A_2(TXA_2) receptor","subitem_subject_scheme":"Other"},{"subitem_subject":"defective TXA_2-induced platelet aggregation","subitem_subject_scheme":"Other"},{"subitem_subject":"G protein","subitem_subject_scheme":"Other"},{"subitem_subject":"phospholipase C","subitem_subject_scheme":"Other"},{"subitem_subject":"point mutation","subitem_subject_scheme":"Other"},{"subitem_subject":"血小板","subitem_subject_scheme":"Other"},{"subitem_subject":"トロンボキサンA_2受容体","subitem_subject_scheme":"Other"},{"subitem_subject":"トロンボキサン不応症","subitem_subject_scheme":"Other"},{"subitem_subject":"G蛋白","subitem_subject_scheme":"Other"},{"subitem_subject":"遺伝子解析","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"トロンボキサンA_2による血小板活性化機構 : トロンボキサン不応症における解析から","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"トロンボキサンA_2による血小板活性化機構 : トロンボキサン不応症における解析から"},{"subitem_title":"トロンボキサンA_2による血小板活性化機構 : トロンボキサン不応症における解析から","subitem_title_language":"en"}]},"item_type_id":"7","owner":"1","path":["456","1114"],"pubdate":{"attribute_name":"公開日","attribute_value":"2017-01-13"},"publish_date":"2017-01-13","publish_status":"0","recid":"16949","relation_version_is_last":true,"title":["トロンボキサンA_2による血小板活性化機構 : トロンボキサン不応症における解析から"],"weko_creator_id":"1","weko_shared_id":null},"updated":"2022-12-15T03:48:24.894777+00:00"}