WEKO3
アイテム
{"_buckets": {"deposit": "099c99eb-799f-4872-ab12-af610f9fbb04"}, "_deposit": {"id": "1667", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "1667"}, "status": "published"}, "_oai": {"id": "oai:niigata-u.repo.nii.ac.jp:00001667", "sets": ["454", "487"]}, "author_link": ["4897", "4898", "4899", "4900"], "control_number": "1667", "item_1627363077551": {"attribute_name": "出版タイプ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_970fb48d4fbd8a85", "subitem_version_type": "VoR"}]}, "item_5_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "1999-02", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "1", "bibliographicPageEnd": "23", "bibliographicPageStart": "18", "bibliographicVolumeNumber": "5", "bibliographic_titles": [{"bibliographic_title": "Food Science and Technology Research", "bibliographic_titleLang": "en"}]}]}, "item_5_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "Two phytase isozymes (PHY1 and PHY2) were purified homogeneously from bran of Triticum aestivum L. cv. Nourin #61 by (NH4)2SO4 fractionation, methanol fractionation, Sephacryl S-200 HR gel filtration chromatography, DEAE-TOYOPEARL chromatography, CM-TOYOPEARL chromatography and second Sephacryl S-200 HR gel filtration chromatography. Molecular weights of the two enzymes were 71,000 and 66,000 by gel filtration, and 68,000 and 66,000 by SDS-PAGE, respectively. Optimum pH and temperatures were 6.0 and 45℃ for PHY1, and 5.5 and 50℃ for PHY2. The activity of both phytases was stable at pHs between pH 4.0-7.0 and below 40℃. The Km values for myo-inositol hexakisphosphate (IHP) were 0.48 μM for PHY1 and 0.77 μM for PHY2. The Ki values for Pi were 2.69 mM for PHY1 and 6.59 mM for PHY2. Both phytases showed relatively high specificity for IHP.", "subitem_description_language": "en", "subitem_description_type": "Abstract"}]}, "item_5_publisher_7": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "日本食品科学工学会", "subitem_publisher_language": "ja"}]}, "item_5_relation_14": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "http://doi.org/10.3136/fstr.5.18", "subitem_relation_type_select": "DOI"}}]}, "item_5_rights_15": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "社団法人日本食品科学工学会"}]}, "item_5_select_19": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_5_source_id_11": {"attribute_name": "書誌レコードID", "attribute_value_mlt": [{"subitem_source_identifier": "AA11320122", "subitem_source_identifier_type": "NCID"}]}, "item_5_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "1344-6606", "subitem_source_identifier_type": "ISSN"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Nakano, Tadao", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "4897", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Joh, Toshio", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "4898", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Tokumoto, Emi", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "4899", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Hayakawa, Toshiro", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "4900", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2019-07-29"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "ZM_5(1)_18-23.pdf", "filesize": [{"value": "837.9 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 837900.0, "url": {"label": "ZM_5(1)_18-23.pdf", "url": "https://niigata-u.repo.nii.ac.jp/record/1667/files/ZM_5(1)_18-23.pdf"}, "version_id": "06db2e2a-ddb7-4c8d-aaac-a22746109758"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "phytase", "subitem_subject_scheme": "Other"}, {"subitem_subject": "phytic acid", "subitem_subject_scheme": "Other"}, {"subitem_subject": "wheat bran", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Nourin #61", "subitem_subject_scheme": "Other"}, {"subitem_subject": "purification", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Purification and Characterization of Phytase from Bran of Triticum aestivum L. cv. Nourin #61", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Purification and Characterization of Phytase from Bran of Triticum aestivum L. cv. Nourin #61", "subitem_title_language": "en"}]}, "item_type_id": "5", "owner": "1", "path": ["454", "487"], "permalink_uri": "http://hdl.handle.net/10191/17598", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2012-04-04"}, "publish_date": "2012-04-04", "publish_status": "0", "recid": "1667", "relation": {}, "relation_version_is_last": true, "title": ["Purification and Characterization of Phytase from Bran of Triticum aestivum L. cv. Nourin #61"], "weko_shared_id": -1}
Purification and Characterization of Phytase from Bran of Triticum aestivum L. cv. Nourin #61
http://hdl.handle.net/10191/17598
http://hdl.handle.net/10191/1759895abd891-acb5-4fe1-a2dc-9d4ce921a206
名前 / ファイル | ライセンス | アクション |
---|---|---|
ZM_5(1)_18-23.pdf (837.9 kB)
|
|
Item type | 学術雑誌論文 / Journal Article(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2012-04-04 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Purification and Characterization of Phytase from Bran of Triticum aestivum L. cv. Nourin #61 | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | phytase | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | phytic acid | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | wheat bran | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Nourin #61 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | purification | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | journal article | |||||
著者 |
Nakano, Tadao
× Nakano, Tadao× Joh, Toshio× Tokumoto, Emi× Hayakawa, Toshiro |
|||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Two phytase isozymes (PHY1 and PHY2) were purified homogeneously from bran of Triticum aestivum L. cv. Nourin #61 by (NH4)2SO4 fractionation, methanol fractionation, Sephacryl S-200 HR gel filtration chromatography, DEAE-TOYOPEARL chromatography, CM-TOYOPEARL chromatography and second Sephacryl S-200 HR gel filtration chromatography. Molecular weights of the two enzymes were 71,000 and 66,000 by gel filtration, and 68,000 and 66,000 by SDS-PAGE, respectively. Optimum pH and temperatures were 6.0 and 45℃ for PHY1, and 5.5 and 50℃ for PHY2. The activity of both phytases was stable at pHs between pH 4.0-7.0 and below 40℃. The Km values for myo-inositol hexakisphosphate (IHP) were 0.48 μM for PHY1 and 0.77 μM for PHY2. The Ki values for Pi were 2.69 mM for PHY1 and 6.59 mM for PHY2. Both phytases showed relatively high specificity for IHP. | |||||
言語 | en | |||||
書誌情報 |
en : Food Science and Technology Research 巻 5, 号 1, p. 18-23, 発行日 1999-02 |
|||||
出版者 | ||||||
言語 | ja | |||||
出版者 | 日本食品科学工学会 | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1344-6606 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA11320122 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | http://doi.org/10.3136/fstr.5.18 | |||||
権利 | ||||||
権利情報 | 社団法人日本食品科学工学会 | |||||
出版タイプ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
著者版フラグ | ||||||
値 | publisher |