@article{oai:niigata-u.repo.nii.ac.jp:00001609,
 author = {Suzuki, Takeshi and Nomura, Shuichi and Maede, Takeyasu and Ohshima, Hayato},
 issue = {3},
 journal = {Cell and tissue research, Cell and tissue research},
 month = {Mar},
 note = {Initial responses of odontoblasts and immunocompetent cells to cavity preparation by laser ablation were investigated in rat molars. In untreated control teeth, an intense heat shock protein (Hsp) 25-immunoreactivity was found in the cell bodies of odontoblasts, whereas cells immunopositive for the class II major histocompatibility complex (MHC) antigen were predominantly located beneath the odontoblast layer in the dental pulp. Cavity preparation caused the destruction of the odontoblast layer and the shift of most class II MHC-positive cells from the pulp-dentin border toward the pulp core at the affected site. Twelve h after cavity preparation, numerous class II MHC-positive cells appeared along the pulp-dentin border and extended their processes deep into the exposed dentinal tubules, but subsequently disappeared from the pulp-dentin border together with Hsp 25-immunopositive cells by 24 h after the operation. By postoperative 3–5 d, a distinct abscess formation consisting of polymorphonuclear leukocytes was found in the dental pulp. The penetration of masses of oral bacteria was recognizable in the dentinal tubules beneath the prepared cavity. These findings indicate that cavity preparation by laser ablation induced a remarkable inflammation by continuous bacterial infections via dentinal tubules in this experimental model that delayed the pulpal regeneration.},
 pages = {311--319},
 title = {An immunocytochemical study of pulpal responses to cavity preparation by laser ablation in rat molars using antibodies to heat shock protein (Hsp) 25 and class II MHC antigen},
 volume = {315},
 year = {2004}
}