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Isolation and Characterization of Microprotoplasts from Propyzamide-treated Cell Suspension Cultures of Hemerocallis hybrida
http://hdl.handle.net/10191/24667
http://hdl.handle.net/10191/246673bacec9d-38f3-4b68-8a37-fa97f4e61ddb
名前 / ファイル | ライセンス | アクション |
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2014-01-08 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Isolation and Characterization of Microprotoplasts from Propyzamide-treated Cell Suspension Cultures of Hemerocallis hybrida | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Hemerocallis hybrida | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | cytochalasin-B | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | micro-nucleus | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | microprotoplast | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | propyzamide | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | journal article | |||||
著者 |
Saito, Hiroyuki
× Saito, Hiroyuki× Nakano, Masaru |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | To produce intergeneric hybrid plants with one or a few chromosomes via microprotoplast fusion in the Liliaceous ornamentals, we developed a system for isolating microprotoplasts from micronucleated cells in suspension cultures of Hemerocallis hybrida cv. Stella d'Oro (2n = 2x = 22). Micronucleation was efficiently induced with a micronucleus index up to 19.1 % by the following sequential treatment of suspension cultures: initially with 2 mM hydroxyurea for 24 h and then with 8 μM propyzamide for 60 h with the addition of 20 μM cytochalasin-B to the cultures 20 h after the initiation of the propyzamide treatment. Following the enzyme treatment of the micronucleated cells and ultra-centrifugation with a continuous iso-osmotic gradient of Percoll solution, microprotoplasts were isolated, each of which had a small nucleus surrounded by a thin rim of cytoplasm. Microprotoplasts less than 10 μm in diameter were obtained with yield of 2.9 × 10^4 cells per 1 ml packed cell volume of suspension cells through sequential filtration with nylon sieves with decreasing pore sizes (50, 20 and 10 μm). The DNA content of the microprotoplasts was less than the 2C level as indicated by flow cytometric analysis, and the relative fluorescence intensity in some of their nuclei corresponded to one or a few chromosomes. | |||||
言語 | en | |||||
書誌情報 |
en : Breeding science 巻 52, 号 1, p. 51-56, 発行日 2002-03 |
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出版者 | ||||||
言語 | ja | |||||
出版者 | 日本育種学会 | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1344-7610 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA11353132 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | http://doi.org/10.1270/jsbbs.52.51 | |||||
権利 | ||||||
権利情報 | 日本育種学会 | |||||
権利 | ||||||
権利情報 | 本文データは学協会の許諾に基づきCiNiiから複製したものである | |||||
出版タイプ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
著者版フラグ | ||||||
値 | publisher | |||||
異版である | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | URI | |||||
関連識別子 | http://ci.nii.ac.jp/naid/110001808122 |