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  1. 0 資料タイプ別
  2. 01 学術雑誌論文
  1. 230 大学院自然科学研究科
  2. 10 学術雑誌論文
  3. 10 査読済論文

Isolation and Characterization of Microprotoplasts from Propyzamide-treated Cell Suspension Cultures of Hemerocallis hybrida

http://hdl.handle.net/10191/24667
http://hdl.handle.net/10191/24667
3bacec9d-38f3-4b68-8a37-fa97f4e61ddb
名前 / ファイル ライセンス アクション
110001808122.pdf 110001808122.pdf (618.3 kB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2014-01-08
タイトル
言語 en
タイトル Isolation and Characterization of Microprotoplasts from Propyzamide-treated Cell Suspension Cultures of Hemerocallis hybrida
言語
言語 eng
キーワード
主題Scheme Other
主題 Hemerocallis hybrida
キーワード
主題Scheme Other
主題 cytochalasin-B
キーワード
主題Scheme Other
主題 micro-nucleus
キーワード
主題Scheme Other
主題 microprotoplast
キーワード
主題Scheme Other
主題 propyzamide
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ journal article
著者 Saito, Hiroyuki

× Saito, Hiroyuki

WEKO 4504

en Saito, Hiroyuki

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Nakano, Masaru

× Nakano, Masaru

WEKO 4505

en Nakano, Masaru

Search repository
抄録
内容記述タイプ Abstract
内容記述 To produce intergeneric hybrid plants with one or a few chromosomes via microprotoplast fusion in the Liliaceous ornamentals, we developed a system for isolating microprotoplasts from micronucleated cells in suspension cultures of Hemerocallis hybrida cv. Stella d'Oro (2n = 2x = 22). Micronucleation was efficiently induced with a micronucleus index up to 19.1 % by the following sequential treatment of suspension cultures: initially with 2 mM hydroxyurea for 24 h and then with 8 μM propyzamide for 60 h with the addition of 20 μM cytochalasin-B to the cultures 20 h after the initiation of the propyzamide treatment. Following the enzyme treatment of the micronucleated cells and ultra-centrifugation with a continuous iso-osmotic gradient of Percoll solution, microprotoplasts were isolated, each of which had a small nucleus surrounded by a thin rim of cytoplasm. Microprotoplasts less than 10 μm in diameter were obtained with yield of 2.9 × 10^4 cells per 1 ml packed cell volume of suspension cells through sequential filtration with nylon sieves with decreasing pore sizes (50, 20 and 10 μm). The DNA content of the microprotoplasts was less than the 2C level as indicated by flow cytometric analysis, and the relative fluorescence intensity in some of their nuclei corresponded to one or a few chromosomes.
言語 en
書誌情報 en : Breeding science

巻 52, 号 1, p. 51-56, 発行日 2002-03
出版者
言語 ja
出版者 日本育種学会
ISSN
収録物識別子タイプ ISSN
収録物識別子 1344-7610
書誌レコードID
収録物識別子タイプ NCID
収録物識別子 AA11353132
DOI
識別子タイプ DOI
関連識別子 http://doi.org/10.1270/jsbbs.52.51
権利
権利情報 日本育種学会
権利
権利情報 本文データは学協会の許諾に基づきCiNiiから複製したものである
出版タイプ
出版タイプ VoR
出版タイプResource http://purl.org/coar/version/c_970fb48d4fbd8a85
著者版フラグ
値 publisher
異版である
関連タイプ isVersionOf
識別子タイプ URI
関連識別子 http://ci.nii.ac.jp/naid/110001808122
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